PeproTech IL-6 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No.	Quantity
500P26GBT50	50 μg
500P26GBT25	25 μg
50-271-8550	500 μg

Catalog No. 500P26GBT50 Supplier PeproTech Supplier No. 500P26GBT50UG

Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human IL-6. Anti-Human IL-6-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-6 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-6 (500-P26G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-6. Western Blot: To detect Human IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P26GBT-1MG will be provided as 2 x 500 μg

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

Specifications

• Antigen: IL-6
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Biotin
• Formulation: PBS with no preservative
• Gene: Il6
• Gene Accession No.: P05231
• Gene Alias: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CHIL-6; CTL differentiation factor; cytokine; HGF; H-IL-6; HSF; hybridoma growth factor; Ifnb2; IFN-beta-2; Il6; IL-6; ILg6; ILN; interferon beta-2; Interleukin; interleukin 6; Interleukin 6 (interferon, beta 2); interleukin 6 precursor; interleukin BSF-2; interleukin HP-1; Interleukin6; Interleukin-6; interleukin-6 precursor; interleukin-6 protein; M-IL-6; prointerleukin 6; R-IL-6
• Gene Symbols: Il6
• Host Species: Goat
• Immunogen: E.coli-derived Recombinant Human IL-6.
• Purification Method: Antigen affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 3569
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized