Novus Biologicals™ IL-6R alpha Antibody Pair [HRP]

Description

Polyclonal/Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-6R alpha.

Specifications

• Assay Range: 6.25 pg/mL
• Assay Sensitivity: 6.25-400 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse IL-6R alpha.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD126, gp80, IL6Q, IL6R, IL-6R-1, IL6RA, IL-6RA, IL6RQ, interleukin 6 receptor
• Gene ID (Entrez): 3570
• Gene Symbol: IL6R
• Host Species: Rabbit
• Kit Contents:
  Standard: Each vial contains 20 ng of recombinant mouse IL-6R alpha.
  Capture Antibody: 0.5 mg/mL of rabbit anti-mouse IL-6R alpha monoclonal antibody,
  Detection Antibody: 0.25 mg/mL rabbit anti-IL-6R alpha polyclonal antibody conjugated to horseradish-peroxidase (HRP).
• Quantity: 15 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: IL-6R alpha
• Technique: 1. Capture Antibody: Dilute to a working concentration of 1 μg/mL in CBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.