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Invitrogen™ IL-8 Human Matched Antibody Pair

Catalog No. 5017943
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10 x 96 Tests
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Catalog No. Quantity
50-179-43 10 x 96 Tests
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Catalog No. 50-179-43 Supplier Invitrogen™ Supplier No. BMS2043MST

Description

Includes

  • 1 vial (5.5mL) monoclonal Coating Antibody to human IL-8/NAP-1 (100μg/mL)
  • 1 vial (55μL) Biotin-Conjugate anti-human IL-8/NAP-1 polyclonalantibody
  • 1 vial (11μL) Streptavidin-HRP
  • 1 vial (30μL) human IL-8/NAP-1 Standard protein, 200ng/mL
  • 2 vials (50mL) Sample Diluent

Matched Antibody Pair

The Human IL-8/NAP-1 Matched Antibody Pair Kit comes with pre-matched antibody pairs,standards,and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-8. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately. Principle of the method ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate,which is an overnight process. Samples,standards,or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody,a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

IL-8 (Interleukin-8) is a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8,secreted by endothelial cells and monocytes,which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8,first isolated from osteosarcoma cells,contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1),granulocyte chemotactic protein 1 (GCP-1),monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
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Specifications

Antigen IL-8
Applications ELISA
Classification Monoclonal/Polyclonal
Conjugate Biotin
Gene Accession No. P10145
Gene Alias Protein 3-10C, CXCL8, NAP-1, GCP-1
Gene Symbols CXCL8
Quantity 10 x 96 Tests
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 3576
Target Species Human
Content And Storage -20°C
Product Type ELISA Kit
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