Invitrogen™ IL1RA Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P209BT500UG

Catalog No. 50-271-8610

Description

AA Sequence of recombinant protein: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1RA. Anti-Human IL-1RA-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1RA (500-P209) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1RA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P209BT-1MG will be provided as 2 x 500 μg

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

Specifications

• Antigen: IL1RA
• Classification: Polyclonal
• Conjugate: Biotin
• Gene: IL1RN
• Gene Alias: Anakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonist
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3557
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P18510
• Gene Symbols: IL1RN
• Immunogen: E.coli-derived Recombinant Human IL-1RA.
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG