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PeproTech IL1RA Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1RA. Anti-Human IL-1RA-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1RA (500-P209) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1RA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P209BT-1MG will be provided as 2 x 500 μg
Specifications
Specifications
| Antigen | IL1RA |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Biotin |
| Formulation | PBS with no preservative |
| Gene | IL1RN |
| Gene Accession No. | P18510 |
| Gene Alias | Anakinra; CD121A; cytokine; D2S1473; DIRA; F630041P17Rik; ICIL-1RA; IL 1 receptor antagonist; IL1 inhibitor; il1 receptor; il-1 receptor; IL1 receptor antagonist; IL1F3; IL1R; il-1r; IL1RA; IL-1RA; IL1-RA; IL-1ra3; IL-1R-alpha; IL1RN; IL-1RN; IL1RN (IL1F3); interleukin 1 receptor antagonist; interleukin-1 receptor; interleukin-1 receptor antagonist; interleukin-1 receptor antagonist protein; interleukin-1 receptor antagonist secretory form; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; IRAP1; MGC10430; MVCD4; P80; type II interleukin-1 receptor antagonist |
| Gene Symbols | IL1RN |
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