Cytiva illustra AutoSeq G-50

Catalog No.	Quantity
45-002-409	1000 Columns

Catalog No. 45-002-409 Supplier Cytiva Supplier No. 27534003

Description

Remove fluorescent dye-terminators from sequencing reactions prior to analysis in an automated sequencer. Cytiva illustra AutoSeq G-50 columns are pre-filled with Sephadex G-50 for effective purification to ensure high-quality sequencing results.

illustra AutoSeq G-50 columns are specifically designed to remove residual fluorescent dye-terminators that can obscure data in automated sequencing. illustra AutoSeq G-50 consists of MicroSpin columns containing Sephadex G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

• For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing
• Columns are prepacked with Sephadex G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative
• Columns that are ready to use enable sample application to collection of purified product in less than 4 minutes
• Can also be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.

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Specifications

• Quantity: 1000 Columns
• Sample Volume: 12 to 25μL