Cytiva blood genomicPrep Mini Spin Kit

Description

Includes

• Spin columns with a novel silica membrane, lyophilized proteinase K powder, lysis solution, wash and elution buffers, microcentrifuge
• collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.

Designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow and nucleated red blood cells.

The purification process involves minimal shearing with typical yields of 4 to 6 μg of genomic DNA per 200μL sample with volumes accommodated up to 1mL. The method uses chaotropic agents to extract DNA from blood cells, denature protein components, and promote the selective binding of DNA to a novel silica-membrane. The elution volume can be modified as necessary for most downstream molecular biological applications.

• High-quality genomic DNA with a greater proportion of higher molecular weight DNA and less shearing
• Demonstrated application to various types of whole blood including human, horse, rabbit, rat and mouse
• Increased ease of use with reduced pipetting volume changes, one centrifugation setting, and color-coded kit components
• Validated in several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests

Specifications

• Description: illustra blood genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high molecular weight genomic DNA (gDNA) from whole blood, buffy coat, bone marrow, and nucleated red blood cells.
• For Use With (Application): Used for several downstream applications including real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digestsSuitable for use in molecular biology applications including standard, multiplex and quantitative PCR; cloning; haplotyping; rest
• Includes: Spin columns prepacked with a novel Silica membrane, Lyophilized Proteinase K powder, Lysis solution, wash and Elution Buffers, microcentrifuge collection tubes, a full protocol booklet and a detachable, quick reference protocol card
• No. of Reactions: 250 Preps
• Product Type: illustra blood genomicPrep Mini Spin Kit
• Purity: 1.7
• Sample Volume: 50 μl whole blood, Buffy coat, bone marrow cells, 2 μl nucleated blood†, 300 μl whole blood‡
• Test Time: < 20 min
• Yield: 4-6 μg/200 μl whole blood