Cytiva GFX 96 PCR Purification Kit

Description

Includes

Binding plates; Wash plates; Collection plates and color-coded bottles of capture buffer; Wash buffer; Choice of two elution buffers (Tris-HCl and sterile water); Instruction booklet

Simultaneously purify up to 96 PCR products of between 100 bp and 10 kbp in as little as 15 minutes

• High yields of pure DNA recovered in a small volume of water or a low-ionic strength buffer
• Typical recoveries are > 85% for PCR products of 100 bp to 10kb length; Salt removal > 99%
• Prepacked glass-fiber matrix 96-well plates are validated for use with the Macherey Nagel NucleoVac 96-vacuum manifold
• No need for ethanol precipitations and hazardous organic extractions

Fluorescent sequencing, microarrays, labeling, hybridization, ligation, and transformation

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Specifications

• Description: Kit uses glass fiber matrix technology in a 96-well format for highly efficient purification of PCR products. DNA fragments from PCR are captured by the matrix in presence of a chaotropic salt and contaminants are removed by washing matrix.
• Sample Type: DNA Solutions, Enzyme Reactions, PCR Mixtures
• For Use With (Application): Fluorescent sequencing, microarrays, labeling, hybridization, ligation and transformation
• Includes: Glass-fiber matrix 96-well plates binding plates, wash plates, collection plates and color-coded bottles of capture Buffer, Wash Buffer choice of two Elution Buffers (Tris HCl and sterile Water), instruction booklet
• Product Type: illustra GFX 96 PCR Purification Kit
• Quantity: 96-Well Plates
• Sample Volume: 100 bp-10 kbp
• Yield: Up to 95% when purifying a 910 bp fragment at a starting concentration of 8.4 ng/μl