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  5. Cytiva Microspin G-50 Columns 

Cytiva Microspin G-50 Columns
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Catalog No. 45001398 Shop All Cytiva Products
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45-001-399 250 Preps
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Catalog No. 45-001-398 Supplier Cytiva Supplier No. 27533001
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Description

Designed to allow the rapid purification of DNA for use in a wide range of applications using typical microfuge equipment. Cytiva illustra™ Microspin G-50 Columns will not remove or denature enzyme.

  • Optimal sample volumes in the range of 12-50μ
  • For rapid purification of labeled DNA (>20 bases) from smaller molecules in solution
  • Columns are prepacked with Sephadex G-50 Grade equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide
  • illustra AutoSeq™ and illustra ProbeQuant™ G-50 Micro Columns are specifically designed for dye terminator removal and labelled probe purification respectively and offer optimized performance in those applications
  • Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 μL) following heat denaturation and phenol extraction
  • Product recovery will be at least 10% lower than that from ProbeQuant G-50 Micro Columns.
  • For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 μl using spin-column chromatography

 

Desalting, buffer exchange; Removal of dye terminators from cycle sequencing reactions; Removal of labeled nucleotides from DNA labeling reactions

Order Info

Conveniently prepacked with Sephadex G-50 DNA Grade pre-equilibrated in TE buffer with 0.05% Kathon™ CG/ICP Biocide and collection tubes

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TRUSTED_SUSTAINABILITY

Specifications

For Use With (Application) Desalting, Buffer exchange, removal of dye terminators from cycle sequencing reactions, removal of labeled, Nucleotides in 25 to 50μL from DNA labeling reactions
Includes Sephadex™ G-50 DNA grade
No. of Reactions 50 Preps
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