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Description
Includes
Fast-flow plasmid 250 columns; Resuspension, lysis, neutralization, wash, elution, and TE buffers; Full protocol booklet with a detachable, quick reference protocol card
The kit consists of a high-performance, anion-exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.
- High column capacity allows for a proportional yield for input sample volume of 25 to 50mL (for high copy number plasmid) and up to 500mL for low copy number plasmid
- Provides plasmid DNA with excellent purity and low endotoxin levels
- Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
Specifications
Specifications
| Description | illustra plasmidPrep Midi Flow Kit is designed for the purification of high yields of transfection-grade plasmid DNA. The kit allows for the purification of large yields of plasmid DNA that are proportional to the input sample volume. |
| For Use With (Application) | Used For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification |
| Includes | Fast-flow genomic 250 columns, lyophilized Proteinase K powder, Lysis solutions, load and Elution Buffers, NAP-25 desalting column, spin column adapters, full protocol booklet, detachable, quick reference protocol card |
| No. of Reactions | 25 Preps |
| Product Type | illustra plasmidPrep Midi Flow Kit |
| Purity | 1.8 |
| Sample Volume | 150 mL Culture |
| Test Time | Approx.215 min |
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