Cytiva Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit

Description

Highly efficient and representative whole-genome amplification with 40 to 60μg yield from nanogram amounts of DNA sample. Cytiva Lifescience™ illustra™ Ready-To-Go™ GenomiPhi V3 DNA Amplification Kit is part of the Phi29 DNA polymerase family of products.

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• Representative amplification of the whole genome.
• Preformulated, predispensed, single-dose, lyophilized cakes for rapid reaction set-up and greater reproducibility.
• Quick and simple automation-friendly protocol; no thermal cycler required.
• Shipping and storage at room temperature provides easy access and reduced carbon footprint.
• Yields high-quality DNA (e.g. for high-throughput genotyping and DNA archival).
• The reaction mixture is provided as predispensed, single-dose, lyophilized cakes in either strips of 8 tubes, or 96-well plates.
• Amplification is highly uniform over the entire genome so that locus representation remains extremely close to the original DNA sample.
• Amplification is carried out with very high fidelity due to Phi29 DNA polymerase proofreading activity.
• The average product length is over 10kb.
• Procedure is quick, simple and does not require a thermocycler.
• A typical DNA yield of 12 to 20μg DNA can be achieved in less than two hours from only 10ng of genomic DNA input.
• The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or a nonpurified cell lysate may be used.
• Protocols for the amplification of DNA from blood lysates and dried blood spots are included in the manual.
• GenomiPhi amplified DNA is suitable for various applications such as genotyping (SNP, STR, array CGH), cloning, sequencing, and DNA archival.

 

Specifications

• Content And Storage: Store the kit at ambient temperature.Once opened, completely reseal the pouch containingthe Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended.Store recons
• Description: illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit offers highly efficient and representative whole-genome amplification with 12 to 20 μg yield from nanogram amounts of DNA sample.
• For Use With (Application): Genomic DNA Amplification
• No. of Reactions: 480
• Product Type: illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit 5x96 Reactions
• Size: > 10 kb
• Test Time: 1.5-2 h