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Cytiva TempliPhi™ 2000 Reaction Kit
GSA_VA

Catalog No. 45003001 Shop All Cytiva Products
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Catalog No. No. of Reactions
45-003-001 2000
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Catalog No. 45-003-001 Supplier Cytiva Supplier No. 28964286
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Description

Includes

TempliPhi Premix, Denature buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications

  • Simple protocol reduces time, labor and consumables needed for template preparation, and workflow enables easy automation
  • Generate microgram quantities of template DNA from picogram amounts of starting material
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks or purified circular (plasmid or M13) DNA
  • Use amplified DNA directly for cycle sequencing without purification
  • Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h

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TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
Description illustra TempliPhi 2000 Reaction Kit
For Use With (Application) High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
Includes TempliPhi™ premix, denature Buffer, Enzyme mix and positive control pUC19 DNA
No. of Reactions 2000
Product Type illustra TempliPhi 2000 Reaction Kit
Test Time 16-18 h
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