Cytiva TempliPhi™ 2000 Reaction Kit

Description

Includes

TempliPhi Premix, Denature buffer, Enzyme mix, and Positive control pUC19 DNA (2ng/μL)

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates with a formulation designed for higher throughput applications

• Simple protocol reduces time, labor and consumables needed for template preparation, and workflow enables easy automation
• Generate microgram quantities of template DNA from picogram amounts of starting material
• Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks or purified circular (plasmid or M13) DNA
• Use amplified DNA directly for cycle sequencing without purification
• Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Content And Storage: Store Denature buffer and control DNA at -70°C or -15°C to -30°C. Store TempliPhi premix at -70°C.Do not store TempliPhi premix at -15°C to -30°C. Thaw the premix on ice and maintain at 0–4°C during handling. Do not allow premix to warm above 4°C prior
• Description: illustra TempliPhi 2000 Reaction Kit
• For Use With (Application): High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.
• Includes: TempliPhi™ premix, denature Buffer, Enzyme mix and positive control pUC19 DNA
• No. of Reactions: 2000
• Product Type: illustra TempliPhi 2000 Reaction Kit
• Test Time: 16-18 h