Cytiva TempliPhi™ Large Construct Kit

Description

Includes

Sample buffer, Reaction buffer, and Enzyme mix

Uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA specifically from large constructs (e.g., fosmids and BACs) sufficient for DNA sequencing

• Start from microliter quantities of purified templates, as well as bacterial culture or glycerol stocks
• Generate 5μg amounts of template DNA for use directly in cycle sequencing reactions without purification
• Simplified process, 20 minutes hands-on time
• Amplification is completed in 18 hours with no need for thermal cycling

Specifications

• Content And Storage: Store the kit at -70°C.The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
• Description: illustra TempliPhi Large Construct Kit uses isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates specifically from large DNA vectors (e.g., fosmids and BACs).
• For Use With (Application): Sequencing Template Amplification Kits and Reagents
• Includes: 9mL Sample Buffer, 9mL Reaction Buffer, 500μL Enzyme mix
• No. of Reactions: 1000
• Product Type: illustra TempliPhi Large Construct Kit
• Test Time: 18 h