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Cytiva IMAC Sepharose™ High Performance Media

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45-000-166 100 mL
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Catalog No. 45-000-166 Supplier Cytiva Supplier No. 17092007
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Description

For optimizing purification of histidine-tagged proteins using Immobilized Metal Affinity Chromatography (IMAC) when Ni2+ is not the best choice of metal ion

  • 34μm beads of highly cross-linked 6% agarose, to which a chelating group has been covalently coupled
  • Charge with transition metal of choice for optimized selectivity
  • Small bead size for high performance purification
  • High binding capacity: ≥40mg histidine-tagged protein/mL medium when charged with Ni2+
  • Allows more sample to be applied; a more concentrated target protein is obtained more at a lower cost/mg and in less time
  • Stable in pH range 3-12 (long term), and in all commonly used buffers

Avoid use with chelating agents, e.g., EDTA, EGTA, or citrate.

TRUSTED_SUSTAINABILITY

Specifications

Flow Rate <300 cm/hr.
pH Range 3 to 12
Particle Size 34 μm
Product Type IMAC Sepharose HP
Content And Storage 4°C to 30°C
Quantity 100 mL
For Use With (Application) Purification of Polyhistidine tagged Proteins
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