Working with live cells often requires sterile working conditions and the continuous maintenance of regulated physiological environments. The Incubator FlowBox has been specifically designed for use with the CellCelector and provides a unique combination of a laminar flow cell culture hood with precise control of temperature humidity and CO2 levels. These features ensure that any environmental changes between the tissue culture incubator and the Incubator FlowBox are kept to an absolute minimum significantly decreasing time constraints to quickly measure identify and transfer cells before temperature or pH changes can impact cellular physiology.