Novus Biologicals™ Influenza A Haemagglutinin H1N1 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Influenza A H1N1 (A/Puerto Rico/8/1934) Haemagglutinin.

Specifications

• Assay Range: 46.88 pg/mL
• Assay Sensitivity: 46.88-3000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Influenza A H1N1 (A/Puerto Rico/8/1934) Haemagglutinin.
• Target Species: Virus
• For Use With (Application): Sandwich ELISA
• Gene Alias: HA, HA1, HA2, Hemagglutinin, Influenza A Virus Hemagglutinin
• Host Species: Mouse
• Kit Contents:
  Standard: Each vial contains 130 ng of recombinant Influenza A Haemagglutinin H1N1.
  Capture Antibody: 0.5 mg/mL of mouse anti-Influenza A Haemagglutinin H1N1 monoclonal antibody (in PBS, pH 7.4).
  Detection Antibody: 0.2 mg/mL mouse anti-Influenza A Haemagglutinin H1N1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4).
• Quantity: 5 Plates
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Target: Influenza A Haemagglutinin H1N1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.