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Invitrogen™ Influenza A M2 Monoclonal Antibody (14C2)
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Catalog No. MA1082
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100 μg
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Catalog No. Quantity
MA1082 100 μg
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Catalog No. MA1082 Supplier Invitrogen™ Supplier No. MA1082
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Description

Mouse Monoclonal Antibody

MA1-082 detects the N-terminal of the influenza A virus M2 protein. MA1-082 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~15 kDa protein representing the M2 protein. MA1-082 has demonstrated the ability to immunoprecipitate M2 from [35s] cysteine-labeled WSN-infected cells. MA1-082 has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain. The MA1-082 immunogen is M2 protein from A/WSN/33-infected CV1 cell lysate.

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
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Specifications

Antigen Influenza A M2
Applications Flow Cytometry, Immunoprecipitation, Western Blot, Immunocytochemistry
Classification Monoclonal
Clone 14C2
Concentration 1 mg/mL
Conjugate Unconjugated
Formulation PBS with 1mg/mL BSA and 0.05% sodium azide
Gene Alias Influenza M2
Host Species Mouse
Immunogen M2 protein from A/WSN/33-infected CV1 cell lysate.
Purification Method Protein A
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Virus
Content And Storage -20°C, Avoid Freeze/Thaw Cycles
Product Type Antibody
Form Liquid
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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