Invitrogen™ iNOS Monoclonal Antibody (M398)

Catalog No.	Quantity
PIMA547726	100 μL

Catalog No. PIMA547726 Supplier Invitrogen™ Supplier No. MA547726

Description

Mouse Monoclonal Antibody

The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNgamma and LPS, or J774A.1 cells treated with LPS only.

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

Specifications

• Antigen: iNOS
• Applications: Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M398
• Concentration: 0.25 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: Nos2
• Gene Accession No.: P29477, P35228, Q06518
• Gene Alias: Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2
• Gene Symbols: Nos2
• Host Species: Mouse
• Immunogen: Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18126, 24599, 4843
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a