Invitrogen™ Integrin beta 7 Monoclonal Antibody (FIB504), eBioscience™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 14586782

Catalog No. 50-132-37

Description

Description: The FIB504 monoclonal antibody reacts with human and mouse integrin beta 7. Integrin beta 7 is a 130 kD glycoprotein which associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Main ligands for integrin alpha 4 beta 7 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the main ligand of integrin alpha E beta 7 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response. The FIB504 antibody has been reported to have blocking activity. Applications Reported: This FIB504 antibody has been reported for use in flow cytometric analysis and functional assays. Applications Tested: This FIB504 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as de...

Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.

Specifications

• Antigen: Integrin beta 7
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P26010, P26011
• Gene Symbols: ITGB7
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16421, 3695
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay
• Clone: FIB504
• Conjugate: Unconjugated
• Gene: ITGB7
• Gene Alias: Gut homing receptor beta subunit; integrin beta 7; integrin beta 7 subunit; integrin beta-7; Integrin beta-P; integrin subunit beta 7; integrin, beta 7; ITGB7; Ly69; M290 IEL antigen
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG2a κ