Invitrogen eBioscience Intracellular Fixation and Permeabilization Buffer (plus Brefeldin A) Kit
Designed for use in preparation of living cells for intracellular staining and flow cytometric analysis. A protein transport inhibitor (Brefeldin A) is included for treatment of cells during culture, to cause accumulation of cytokine protein at the Golgi and enhance resulting staining.
Manufacturer: Invitrogen 88882388
- Fixation buffer is used to cross-link, stabilize, and fix the cell membrane
- Permeabilization buffer is used to reversibly permeabilize the cell membrane so the staining antibodies can enter the cell effectively
|Intracellular Staining Followed by Flow Cytometric Analysis|
|2° to 8°C|
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