Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
High-specificity, high-yield PCR amplification of GC-rich templates
Manufacturer: Invitrogen™ 12337024
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B).
- Yield: high yields for targets up to 5kb in length
- Specificity: AccuPrime accessory proteins for improved PCR specificity
- Sensitivity: sensitivity down to 5ng of template DNA
- Robust and specific amplifications
- Built-in hot start
The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.
One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10nmoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Amplification of Bisulfite-Treated DNA, GC-Rich PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research
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|• 1 x 500 μL AccuPrime GC-Rich DNA Polymerase (2U/μL)
• 1 x 5 mL 5X AccuPrime GC-Rich Buffer A
• 1 x 5 mL 5X AccuPrime GC-Rich Buffer B
• 1 x 1 mL 50 mM MgSO4
Store at -20°C.
|Built-In Hot Start|
|5 kb or less|
|Approved for shipment on Wet or Dry Ice|
|AccuPrime™ GC-Rich DNA Polymerase|
|3' - 5'|
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For Research Use Only. Not for use in diagnostic procedures.