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Invitrogen™ AccuPrime™ Pfx DNA Polymerase

Provides higher fidelity than Pfu DNA polymerase

Manufacturer:  Invitrogen™ 12344032

Encompass

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Catalog No. 12-344-032


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Includes: AccuPrime Pfx DNA Polymerase (100μL, at 2.5U/μL) is provided with 10X AccuPrime reaction mix (1mL) that contains dNTPs and a separate vial (1mL) of 50mM MgSO4. Reagents are provided for 200 amplification reactions of 50μL each.

Description

Description

AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcusspecies KODwith proofreading (3'-5' exonuclease) activity. Platinumanti-PfxDNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity. Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over PlatinumPfx alone.

  • Achieve greater than 26 times higher fidelity than Taq DNA polymerase
  • Amplify fragments up to 12 kb
  • Minimize PCR optimization
  • Built-in hot start

Applications
Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

• 1 x 500 μL AccuPrime Pfx DNA Polymerase (2.5 U/μL)
• 5 x 1 mL 10X AccuPrime Pfx Reaction Mix
• 2 x 1 mL 50 mM MgSO4

Store at -20°C.
26 X
Low
1000 Reactions
AccuPrime™ Pfx DNA Polymerase
Standard
DNA
3' - 5'
1000 Reactions
Primer-Probe
Stand-alone enzyme
Built-In Hot Start
Blunt
Separate Components
12 kb or less
500 μL
AccuPrime™
Approved for shipment on Wet or Dry Ice
SDS
Documents

Documents

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For Research Use Only. Not for use in diagnostic procedures.