Invitrogen™ AccuPrime™ Pfx SuperMix

High-fidelity, high-specificity amplification of DNA fragments

Manufacturer:  Invitrogen™ 12344040

Catalog No. 12-344-040

Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO₄ (pH 8.4), 30.8mM (NH₄)₂SO₄, 11mM KCl, 1.1mM MgSO₄, 330μM dNTPs, AccuPrime proteins, stabilizers.

Description

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half.

• Achieve >26-times higher fidelity than with Taq DNA polymerase
• Amplify fragments up to 12kb
• Mminimize PCR optimization
• Produce products with blunt ends
• Built-in hot start

Using AccuPrime Pfx SuperMix
AccuPrime Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

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Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Content And Storage: • 4 × 1.125 mL AccuPrime Pfx SuperMix
  
  Store at -20°C in a non-frost-free freezer.
• Detection Method: Primer-Probe
• Format: SuperMix or Master Mix
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 15 kb or less
• Exonuclease Activity: 3' - 5'
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• For Use With (Application): PCR Amplification

• Concentration: 1.1X
• Fidelity (vs. Taq): 26 X
• GC-Rich PCR Performance: Low
• No. of Reactions: 200 Reactions
• Polymerase: AccuPrime™ Pfx DNA Polymerase
• Reaction Speed: Standard
• Starting Material: DNA
• Product Line: AccuPrime™
• Sufficient For: 200 Reactions

For Research Use Only. Not for use in diagnostic procedures.