Invitrogen™ AccuPrime™ Pfx SuperMix

Description

Includes

AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO₄ (pH 8.4), 30.8mM (NH₄)₂SO₄, 11mM KCl, 1.1mM MgSO₄, 330μM dNTPs, AccuPrime proteins, stabilizers.

Requires

Template and primers

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.

Features

• >26-times higher fidelity than with Taq DNA polymerase
• Reduced set-up time
• Produces products with blunt ends

Applications

• High-fidelity PCR
• Amplification of fragments up to 15 kb
• Cloning & mutagenesis

Notes

• Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Concentration: 1.1X
• Content And Storage: AccuPrime Pfx SuperMix (4 × 1.125 mL)
  
  Store at –20°C in a non-frost-free freezer.
• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Polymerase: AccuPrime Pfx DNA Polymerase
• Reaction Speed: Standard
• Quantity: 200 rxns
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Hot-start PCR, High-fidelity PCR
• Fidelity (vs. Taq): 26X
• Hot Start: Built-In Hot Start
• No. of Reactions: 200 Reactions
• Overhang: Blunt
• Reaction Format: SuperMix or Master Mix
• Size (Final Product): 15 kb or less

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.