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Invitrogen™ AccuPrime™ Pfx SuperMix Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

High-fidelity, high-specificity amplification of DNA fragments

Manufacturer:  Invitrogen™ 12344040

Catalog No. 12-344-040


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Includes: AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25µL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330µM dNTPs, AccuPrime proteins, stabilizers.

Description

Description

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half.

  • Achieve >26-times higher fidelity than with Taq DNA polymerase
  • Amplify fragments up to 12kb
  • Mminimize PCR optimization
  • Produce products with blunt ends
  • Built-in hot start

Using AccuPrime Pfx SuperMix
AccuPrime Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. Thermostable AccuPrime proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research

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Order Info

Approved for shipment on Wet or Dry Ice

Specifications

Specifications

200 reactions
Fidelity (vs. Taq): 26X
AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.
Store at -20°C in a non-frost-free freezer.
Gel Compatibility: Low
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For Research Use Only. Not for use in diagnostic procedures.