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Invitrogen™ AccuPrime™ Pfx SuperMix

Catalog No. 12344040
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Catalog No. 12-344-040 Supplier Invitrogen™ Supplier No. 12344040
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Description

Includes

AccuPrime Pfx SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25μL each (4 x 1.125mL) are provided. AccuPrime Pfx SuperMix Components: 22U/mL Thermococcus species KOD thermostable polymerase complexed with anti-KOD antibodies, 66mM Tris-SO4 (pH 8.4), 30.8mM (NH4)2SO4, 11mM KCl, 1.1mM MgSO4, 330μM dNTPs, AccuPrime proteins, stabilizers.

Requires

Template and primers

AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.

Invitrogen AccuPrime Pfx SuperMix provides reagents for high-fidelity, high-specificity amplification of DNA fragments. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading activity. This ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs is designed for efficient PCR amplification.

Features

  • >26-times higher fidelity than with Taq DNA polymerase
  • Reduced set-up time
  • Produces products with blunt ends

Applications

  • High-fidelity PCR
  • Amplification of fragments up to 15 kb
  • Cloning & mutagenesis

Notes

  • Thermostable AccuPrime Pfx SuperMix proteins enhance primer-template hybridization during every cycle, increasing specificity and yield.

Order Info

Approved for shipment on Wet or Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 1.1X
Content And Storage AccuPrime Pfx SuperMix (4 × 1.125 mL)

Store at –20°C in a non-frost-free freezer.
Detection Method Primer-probe
GC-Rich PCR Performance Low
Polymerase AccuPrime Pfx DNA Polymerase
Reaction Speed Standard
Quantity 200 rxns
Shipping Condition Wet or Dry Ice
For Use With (Application) Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq) 26X
Hot Start Built-In Hot Start
No. of Reactions 200 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Size (Final Product) 15 kb or less
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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