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Invitrogen™ AccuPrime™ Taq DNA Polymerase, high fidelity

Amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

Supplier:  Invitrogen™ 12346094

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Catalog No. 12-346-094

  • $2,190.65 / Each of 1

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    Reg : $2,355.00

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Includes: AccuPrime™ Taq DNA Polymerase High Fidelity (40μL, at 5U/μL)) is supplied with: 50mM MgSO4 (1mL), 10X AccuPrime™ PCR Buffer I (1mL), 10X AccuPrime™ PCR Buffer II (1mL)

Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600mM Tris-SO4 (pH 8.9), 180mM (NH4)2SO4, 20mM MgSO4, 2mM dGTP, 2mM dATP, 2mM dTTP, 2mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λDNA. Buffer II is optimized for genomic DNA.

Description

Description

  • High specificity and yield for the most robust PCR amplification
  • 9-fold higher fidelity than TaqDNA polymerase alone
  • Mimimal optimization steps, even with nonoptimized primer sets
  • Efficient amplification of targets over a broad size range up to 20kb
How it work
High fidelity is achieved by a combination of Platinum antiTaqDNA polymerase antibodies that inhibit polymerase activity, providing an automatic “hot-start”, and the proofreading (3'-5' exonuclease activity) enzyme Pyrococcusspecies GB-D. The thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield.

Amplification of Bisulfite-Treated DNA, High Fidelity PCR, Hot Start PCR, Long Fragment PCR, Methylation Analysis, PCR, PCR & Real-Time PCR, RNAi, Epigenetics & Noncoding RNA Research

Order Info

Shipping Condition: Dry ice

TRUSTED_SUSTAINABILITY
Specifications

Specifications

• 1 x 200 μL AccuPrime Taq DNA Polymerase, High Fidelity (5 U/μL)
• 4 × 1.25 mL 10X AccuPrime PCR Buffer I
• 4 × 1.25 mL 10X AccuPrime PCR Buffer II
• 1 x 1 mL 50 mM MgSO4

Store at -10°C to -30°C.
Tube
AccuPrime Taq DNA Polymerase High Fidelity
3' - 5', 5' - 3'
DNA Polymerase
Dry Ice
9 X
1000 Reactions
Separate Components
DNA
Primer-probe
Low
Standard
AccuPrime™
1000 Reactions
1000 Reactions
Built-In Hot Start
Mixed, 3'-A
20 kb or less
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For Research Use Only. Not for use in diagnostic procedures.