Invitrogen™ AccuPrime™ Taq DNA Polymerase System  

Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications

Manufacturer:  Invitrogen™ 12339016

Catalog No. 12-339-016

Description

The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases. Platinumanti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, providing optimal performance between 55°C and 65°C.

• Specificity: Control mispriming at every cycle to get single-band PCR products
• Ease of use: minimize reaction optimization and primer set redesign
• Convenience: conveniently assemble reactions at room temperature

• AccuPrime buffers: 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations
• Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
• For small genomic DNA amplicons (≤200bp), cDNA, or plasmids

Amplification of Bisulfite-Treated DNA, Hot Start PCR, Methylation Analysis, Multiplex PCR, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Non-Coding RNA Research, Routine PCR

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Specifications

• Concentration: 10 X
• Detection Method: Primer-Probe
• Fidelity (vs. Taq): 2 X
• GC-Rich PCR Performance: Low
• No. of Reactions: 200
• Overhang: 3'-A
• Polymerase: AccuPrime™ Taq DNA Polymerase
• Product Type Specs: DNA Polymerase System
• Reaction Speed: Standard
• Size (Final Product): 5 kb or less

• Content And Storage: AccuPrime™ Taq DNA Polymerase is provided in a 200 reaction kit. Each kit includes:
  
  • AccuPrime™ Taq DNA Polymerase (100 µL, at 2U/µL)
  
  • 10X AccuPrime™ PCR Buffer I* (500 µL)
  
  • 10X AccuPrime™ PCR Buffer II* (500 µL)
  
  • 50 mM Magnesium Chloride (500 µL)
  
  *10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).
  
  10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl₂, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.
  
  Store at -20°C.
• Exonuclease Activity: 5' - 3'
• For Use With (Application): PCR Amplification
• Hot Start: Built-In Hot Start
• No. of Tests: 200 Reactions
• PCR Method: Multiplex PCR, RT-PCR
• Product Line: AccuPrime™
• Reaction Format: Separate Components
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Sufficient For: 200 Reactions

For Research Use Only. Not for use in diagnostic procedures.