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Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)

Catalog No. AM8439
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AM8439 50 μL
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Catalog No. AM8439 Supplier Invitrogen™ Supplier No. AM8439
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Description

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups.

When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.

Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.

Order Info

Shipping Conditions: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Concentration 50 mM
Content And Storage 5-(3-Aminoallyl)-dUTP, 50 mM (50 μL)

Store at –70°C.
Form Liquid
Label or Dye Aminoallyl
Product Line Ambion
Product Type Nucleotides
Purification Method HPLC
Quantity 50 μL
Shipping Condition Dry Ice
For Use With (Application) Nucleic Acid Labeling

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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