Invitrogen™ 5-(3-Aminoallyl)-dUTP (50 mM)
When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups
Supplier: Invitrogen™ AM8439
The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
- Use of this Modified Nucleotide: Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription
- Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals
Gene Expression Analysis & Genotyping, in vitro Transcription
Shipping Conditions: Dry Ice
|Store at or below -70°C. Avoid multiple freeze-thaw cycles. Aliquots of the product may be stored short-term at -20°C. Do not store in a frost-free freezer.|
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For Research Use Only. Not for use in diagnostic procedures.