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Invitrogen™ Bacterial Alkaline Phosphatase Non-distribution product as customer accommodation.

Removes 3' and 5' phosphates from DNA and RNA

Manufacturer:  Invitrogen™ 18011015

Catalog No. 18-011-015


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Includes: A vial of 10X dephosphorylation buffer [100mM Tris-HCl (pH 8.0)].

Description

Description

BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction

  • Source: Purified from E. coli C90
  • Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
  • Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
  • Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-32P]ATP, and enzyme in 50µL for 30 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

Order Info

Shipping Conditions: Wet ice

Specifications

Specifications

10X Dephosphorylation Buffer [100mM Tris-HCl (pH 8.0)]
2,500U
Store at -20°C
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For Research Use Only. Not for use in diagnostic procedures.