Invitrogen Bacterial Alkaline Phosphatase
Removes 3' and 5' phosphates from DNA and RNA
Manufacturer: Invitrogen 18011015
BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction
- Source: Purified from E. coli C90
- Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
- Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
- Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-32P]ATP, and enzyme in 50µL for 30 min. at 37°C
Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning
Shipping Conditions: Wet ice
|10X Dephosphorylation Buffer [100mM Tris-HCl (pH 8.0)]|
|Store at -20°C|
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For Research Use Only. Not for use in diagnostic procedures.