Invitrogen™ Bacterial Alkaline Phosphatase

Catalog No.	Quantity
18-011-015	2500 U

Catalog No. 18-011-015 Supplier Invitrogen™ Supplier No. 18011015

Description

Includes

A vial of 10X dephosphorylation buffer [100mM Tris-HCl (pH 8.0)].

Removes 3' and 5' phosphates from DNA and RNA

BAP is active at 65°C for at least 1 hr. and is inactivated by phenol extraction

• Source: Purified from E. coli C90
• Performance and Quality Testing: Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency determined
• Unit Definition: One unit hydrolyzes 1nmol of ATP in 30 min. at 37°C
• Unit Reaction Conditions: 10mM Tris-HCl (pH 8.0), 1.5mM [γ-³²P]ATP, and enzyme in 50μL for 30 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

Order Info

Shipping Conditions: Wet ice

Specifications

• Content And Storage: Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C.
• Shipping Condition: Wet Ice
• Enzyme: BAP
• Quantity: 2500 U
• Product Type: Alkaline Phosphatase

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.