CIAP (Calf Intestinal Alkaline Phosphatase) Non-distribution product as customer accommodation.

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Manufacturer: Invitrogen 18009019

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Catalog No. 18-009-019

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    Specifications

    Concentration 20U/μL
    Includes 10X Dephosphorylation Buffer [500mM Tris-HCl (pH 8.5), 1mM EDTA] and Dilution Buffer [50% glycerol, 25mM Tris-HCl (pH 7.6), 1mM MgCl2 , and 0.1mM ZnCl2]
    Quantity 1000 units
    Storage Requirements Store at -20°C

    • Concentration: 20 units/µL
    • Source: Purified from calf intestinal mucosa
    • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
    • Unit Definition: One unit hydrolyzes 1µmol of 4-nitrophenyl phosphate in 1 min. at 37°C
    • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900µL for 10 min. at 37°C

    Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

  • Description & Specifications

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