Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL

Description

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

• Concentration: 20 units/μL
• Source: Purified from calf intestinal mucosa
• Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
• Unit Definition: One unit hydrolyzes 1μmol of 4-nitrophenyl phosphate in 1 min. at 37°C
• Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl₂ (pH 9.8) in 900μL for 10 min. at 37°C

Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

Specifications

• Concentration: 20 U/μL
• Content And Storage: Calf Intestinal Alkaline Phosphatase (20 units/μL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl₂ , and 0.1 mM ZnCl₂ ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Enzyme: CIP
• Quantity: 1000 U
• Product Type: Alkaline Phosphatase

For Research Use Only. Not for use in diagnostic procedures.