CIAP (Calf Intestinal Alkaline Phosphatase) Non-distribution product as customer accommodation.

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Manufacturer: Invitrogen 18009027

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Catalog No. 18-009-027

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    Concentration 1U/μL
    Quantity 1000 units
    Storage Requirements Store at -20°C

    • Concentration: 20 units/µL
    • Source: Purified from calf intestinal mucosa
    • Performance and Quality Testing: Endodeoxyribonuclease, 3' exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay
    • Unit Definition: One unit hydrolyzes 1µmol of 4-nitrophenyl phosphate in 1 min. at 37°C
    • Unit Reaction Conditions: 1M diethanolamine buffer, 10mM 4-nitrophenyl phosphate, 0.25mM MgCl2 (pH 9.8) in 900µL for 10 min. at 37°C

    Dephosphorylation of 5'-phosphorylated termini of vector DNA to prevent self-ligation; Dephosphorylation of 5' termini of nucleic acids prior to forward reaction with kinase; Cloning; Restriction Enzyme Cloning

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