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Invitrogen™ Countess™ Cell Counting Chamber Slides and Holder, disposable
Description
Includes
Sildes and 20 × 1mL vials of trypan blue (0.4%)
Countess Cell Counting Chamber Slides are designed exclusively for automated cell counting on the Countess Automated Cell Counter. These individually packaged slides feature two enclosed chambers and are disposable, offering convenience when performing cell culture. The Countess family of automated cell counters employs the widely used trypan blue staining method for cell counting to identify dead cells and measure cellular viability using brightfield.
The disposable Countess Cell Counting Chamber Slides offer convenience in automated cell counting for processing multiple cell samples, busy cell culture facilities, and easy disposal of contaminated or hazardous samples. They also eliminate the risk of contamination from residual samples. After use, Countess slides can be disposed of as standard biohazard waste. Unlike a hemocytometer, there is no need to clean Countess slides before or after use.
Each Countess Cell Counting Chamber Slide has two separate, enclosed chambers for counting one sample in duplicate or two different samples. Cell counting occurs in a central location of the counting chamber. The volume counted is 0.4 μL, the same as counting four 1-mm2 squares in a standard hemocytometer.
The Countess Automated Cell Counter works in three simple steps:
- Mix 10 μL of sample with 10 μL of trypan blue stain or other desired reagent, including ReadyCount™ stains, and pipet into a disposable Countess chamber slide
- Insert the slide into the instrument
- Press 'Count'
That's it—get the total cell count, percent viable cells, and average cell size in as little as 30 seconds.
The Countess Cell Counting Chamber Slides include two vials of trypan blue for brightfield automated cell counting and viability analysis (Cat. Nos. C10228, C10312, C10314, C10313, and C10315). The slides can also be purchased as 1 box of 50 slides without trypan blue (Cat. No. C10283) for use with other stains such as the ReadyCount fluorescent stains. The trypan blue vials are also available for purchase without slides (Cat. No. T10282).
Trypan blue staining is a long-standing and widely used method to measure cellular viability by identifying dead cells. Live cells with intact membranes exclude the dye, while dead cells with compromised membranes become stained, allowing for the differentiation and counting of live and dead cells to assess cellular viability. Trypan blue staining is the method employed by the Countess family of automated cell counters for cell counting, and this 0.4% trypan blue solution is the correct concentration for use on these instruments.
Note: Precipitates are quite common in trypan blue, and typically increase with storage time and freezing. The three approaches below may help minimize trypan blue precipitate in your cell counting samples:
- Sterile filtration of trypan blue using 0.3-micron filter
- Centrifugation or passive settling (avoid mixing or vortexing trypan stock solutions)
- Gentle warming of the 1-mL trypan solution to 37°C for 10 minutes
The Countess Disposable Slide Holder is used to hold the Countess disposable slides. It is designed for use with Countess II FL, Countess 3, and Countess 3 FL automated cell counters and is included with these cell counters. A replacement Countess Disposable Slide Holder (Cat. No. AMEP4745 ) is available for separate purchase. This Countess Disposable Slide Holder is not compatible with the Countess Reusable Slide (Cat. No. A25750).
Specifications
Specifications
| Type | Cell Counting Chamber Slide |
| Product Line | Countess |
| For Use With (Equipment) | Countess Automated Cell Counter |
| Holds | 10 μL/Chamber |
| Positions | 2 Chambers/Slide |
| Depth (Metric) | 100 μm (Chamber) |
| Dimensions | 75 x 25 x 1.8 mm (Chamber) |
| Content And Storage | • Countess™ Cell Counting Chamber Slides, 1 x 50 slides • Trypan blue stain, 2 x 1 mL vials Each slide contains two cell counting chambers. For use at room temperature under normal laboratory conditions. |
| Quantity | 50 Slides |
Frequently Asked Questions (FAQs)
Yes. The alternative options for Countess Cell Counting Chamber Slides (Cat. No. C10283) and for Countess 3 and Countess 3 FL Automated Cell Counter can be found using the link below:
Countess Cell Counting Chamber Slides (Cat. Nos. C10228, C10312, C10313, C10314, C10315)
The Countess Cell Counting Chamber Slides (Cat. No. C10228, C10312, C10314, C10315) are compatible with all Countess Automated Cell Counter instruments.
The Countess II and Countess II FL instruments come precalibrated. You do not have to calibrate the instruments.
It is based on dye exclusion using 0.2% Trypan Blue dye. Trypan Blue is excluded by the live cells and enters the dead cells; this results in intense staining of the intracellular proteins.
The following cell lines and primary cells have been tested:
- Cell lines: HEK 293, A431, CHO-M1, CHSE, COLO-205, COS-7, HeLa, HepG2, HL-60, J774(MMM), Jurkat, K-562, MCF-7, MRC-5, NIH/3T3, PC-12, SF-21, U266, and U2OS
- Primary cells: Human adipose tissue-derived stem cells, HASMCs, HPAECs, HPASMCs, HUVECs, C2C12, RBCs, and PMBCs
We are continually testing additional cell types.
For Research Use Only. Not for use in diagnostic procedures.
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