Invitrogen™ MegaX DH10B T1^R Electrocomp™ Cells  

Highest-efficiency electrocompetent cells available

Manufacturer:  Invitrogen™ C640003

Catalog No. C640003

Includes: Contains: MegaX DH10B™ T1R Electrocomp™ C: 5 vials, 100 µL each; pUC19 DNA (10pg/µL): 1 vial, 50µL; Recovery Medium: 3 bottles, 10mL each

Description

Improved production processes guarantee a three-fold greater number of colonies per transformation (>3 x 10¹⁰ cfu/µg of pUC DNA), making the MegaX DH10B™ T1^R cells ideal for highly demanding cloning and library construction. Same genotype as the widely used DH10B™ T1^R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards valuable clones and libraries.

• Provide highly efficient transformation of methylated DNA, since they lack E. coli K12 restriction systems
• ψ80lacZδM15 marker provides α-complementation of β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal
• Bacterial/Yeast Strain: DH10B™

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

• Bacterial or Yeast Strain: DH10B
• Cloning Methylated DNA: Yes
• Competent Cell Type: Electrocompetent
• F'episome (to make ssDNA): Lacks F' Episome
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Plasmid: May be used for plasmids > 100 kb
• Product Line: DH10B, Electrocomp™
• Reduces Recombination: Yes
• Species: E. coli
• Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)

• Blue/White Screening: Yes
• Cloning Unstable DNA: Not Suitable for cloning unstable DNA
• Content And Storage: Contains:
  • MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each
  • pUC19 DNA (10 pg/µl): 1 vial, 50 µl
  • Recovery Medium: 3 bottles, 10 ml each
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .
• Format: Tube(s)
• Improves Plasmid Quality: Yes
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Transformation Efficiency: > 3x10^10

For Research Use Only. Not for use in diagnostic procedures.