Invitrogen MegaX DH10B T1R Electrocomp Cells
Highest-efficiency electrocompetent cells available
Manufacturer: Invitrogen C640003
Improved production processes guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/µg of pUC DNA), making the MegaX DH10B T1R cells ideal for highly demanding cloning and library construction. Same genotype as the widely used DH10B T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards valuable clones and libraries.
- Provide highly efficient transformation of methylated DNA, since they lack E. coli K12 restriction systems
- ψ80lacZδM15 marker provides α-complementation of β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal
- Bacterial/Yeast Strain: DH10B
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Shipping Condition: Dry Ice
|Lacks F' Episome|
|Not High-throughput Compatible (Manual)|
|May be used for plasmids > 100 kb|
|High Efficiency (> 10^9 cfu⁄µg)|
|Not Suitable for cloning unstable DNA|
• MegaX DH10B T1R Electrocomp C: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• Recovery Medium: 3 bottles, 10 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store Recovery Medium at 4°C .
|Not Suitable for preparing unmethylated DNA|
|Not for ccdB vector propagation|
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For Research Use Only. Not for use in diagnostic procedures.