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Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Highest-efficiency electrocompetent cells available

Manufacturer:  Invitrogen™ C640003

Catalog No. C640003


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Includes: Contains: MegaX DH10B™ T1R Electrocomp™ C: 5 vials, 100 μL each; pUC19 DNA (10pg/μL): 1 vial, 50μL; Recovery Medium: 3 bottles, 10mL each

Description

Description

Improved production processes guarantee a three-fold greater number of colonies per transformation (>3 x 1010 cfu/μg of pUC DNA), making the MegaX DH10B™ T1R cells ideal for highly demanding cloning and library construction. Same genotype as the widely used DH10B™ T1R strain, including tonA, which prevents infection by T1 and T5 lytic bacteriophages, and safeguards valuable clones and libraries.

  • Provide highly efficient transformation of methylated DNA, since they lack E. coli K12 restriction systems
  • ψ80lacZδM15 marker provides α-complementation of β-galactosidase gene, allowing blue/white screening on agar plates containing X-gal or Bluo-gal
  • Bacterial/Yeast Strain: DH10B™

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Yes
Electrocomp Cell
Yes
Not Suitable for cloning unstable DNA
High Efficiency (> 10^9 cfu⁄µg)
Not Suitable for preparing unmethylated DNA
E. coli
Yes
Not High-throughput Compatible (Manual)
Not for ccdB vector propagation
May be used for plasmids > 100 kb
Transformation, Cloning
Dry Ice
DH10B
> 3x10^10
Yes
Yes
Lacks F' Episome
DH10B, Electrocomp™
Tube(s)
Electrocompetent
Safety and Handling

Safety and Handling

SDS
Documents
Product Certifications
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For Research Use Only. Not for use in diagnostic procedures.