Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ DH10Bac™ MAX Efficiency™ MAX Efficiency DH10Bac Competent Cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

Manufacturer:  Invitrogen™ 10361012

Catalog No. 10-361-012


Add to cart

Includes: MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100µL each (total of 500µL), pUC19 DNA (0.01 µg/mL): 1 vial, 100µL, SOC Medium: 2 bottles, 6mL each

Description

Description

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac™ Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest.

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac™ vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and can generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124

  • Consistent yields of >1 x 108 transformants/µg
  • Tolerance of small amounts of undiluted ligation reactions
  • Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Not High Throughput-Compatible (Manual)

Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Chemically Competent
MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100μL each (total of 500μL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, SOC Medium: 2 bottles, 6mL each
> 1x108
No
No
Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
DH10Bac
No
No
Yes
E. coli
5 x 100μL
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.