Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Description

Includes

MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100μL each (total of 500μL), pUC19 DNA (0.01 μg/mL): 1 vial, 100μL, SOC Medium: 2 bottles, 6mL each

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac™ Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest.

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac™ vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and can generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124

• Consistent yields of >1 x 10⁸ transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Not High Throughput-Compatible (Manual)

Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Competent Cells
• Contains F' Episome: Lacks F' Episome
• Efficiency: > 1x10^8
• Cell Type: Chemically Competent
• Improves Plasmid Quality: No
• Cloning Methylated DNA: No
• Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄μg)
• Content And Storage: Contains:
  • MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)
  • pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl
  • SOC Medium: 2 bottles, 6 ml each
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• Cloning Unstable DNA: Not Suitable for cloning unstable DNA
• Bacterial or Yeast Strain: DH10Bac
• Blue/White Screening: Yes
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Reduces Recombination: No
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli
• Format: Tube
• Product Line: DH10Bac, MAX Efficiency™
• Quantity: 5 x 100 μL

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.