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Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Catalog No. 10361012
Encompass_Preferred
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Quantity:
5 x 100 μL

Includes

MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100μL each (total of 500μL), pUC19 DNA (0.01 μg/mL): 1 vial, 100μL, SOC Medium: 2 bottles, 6mL each

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac™ Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest.

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac™ vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and can generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124

  • Consistent yields of >1 x 108 transformants/μg
  • Tolerance of small amounts of undiluted ligation reactions
  • Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Not High Throughput-Compatible (Manual)

Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation

Order Info

Shipping Condition: Dry Ice

TRUSTED_SUSTAINABILITY

Specifications

Product Type Competent Cells
Contains F' Episome Lacks F' Episome
Efficiency > 1x10^8
Cell Type Chemically Competent
Improves Plasmid Quality No
Cloning Methylated DNA No
Transformation Efficiency Level Medium Efficiency (10^8-10^9 cfu⁄μg)
Content And Storage Contains:
• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)
• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl
• SOC Medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Cloning Unstable DNA Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain DH10Bac
Blue/White Screening Yes
High-throughput Compatibility Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors Not for ccdB vector propagation
Reduces Recombination No
Shipping Condition Dry Ice
T1 Phage - Resistant (tonA) No
Species E. coli
Format Tube
Product Line DH10Bac, MAX Efficiency™
Quantity 5 x 100 μL
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WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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