Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells  

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

Manufacturer:  Invitrogen™ 10361012

Catalog No. 10-361-012

Includes: MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100μL each (total of 500μL), pUC19 DNA (0.01 μg/mL): 1 vial, 100μL, SOC Medium: 2 bottles, 6mL each

Description

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac™ Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac™, to create an expression bacmid containing a cloned gene of interest.

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac™ vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and can generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124

• Consistent yields of >1 x 10⁸ transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Not High Throughput-Compatible (Manual)

Baculovirus Production and Titering, Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

• Bacterial or Yeast Strain: DH10Bac
• Cloning Methylated DNA: No
• Competent Cell Type: Chemically Competent
• F'episome (to make ssDNA): Lacks F' Episome
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)

• Blue/White Screening: Yes
• Cloning Unstable DNA: Not Suitable for cloning unstable DNA
• Content And Storage: Contains:
  • MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 µl each (total of 500 µl)
  • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
  • SOC Medium: 2 bottles, 6 ml each
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• Format: Tube(s)
• Improves Plasmid Quality: No
• Product Line: DH10Bac, MAX Efficiency™
• Reduces Recombination: No
• Species: E. coli
• Transformation Efficiency: > 1x10^8

For Research Use Only. Not for use in diagnostic procedures.