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Invitrogen™ DNA Polymerase I/DNase I Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

An optimized mixture of both enzymes for efficient nick translation of DNA

Manufacturer:  Invitrogen™ 18162016

Catalog No. 18-162-016


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Includes: DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter.

Description

Description

  • Application: Labeling DNA with either radiolabeled or biotinylated nucleotides
  • Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen nM 964
  • Performance and Quality Testing: Performance tested in nick translation reaction
  • Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10nMol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template primer
  • Unit Reaction Conditions: 50mM potassium phosphate (pH 7.5), 6.7mM MgCl2 , 1mM 2-mercaptoethanol, 80µg/mL template·primer, 32µM dTTP, 69nM [3H]dTTP, and enzyme in 100µL for 30 min. at 37°C

Cloning, Restriction Enzyme Cloning

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Order Info

Shipping Condition: Wet Ice

Specifications

Specifications

Enzymes, DNA Polymerase I/DNase I
DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter
Cloning, Restriction Enzyme Cloning
250 units
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For Research Use Only. Not for use in diagnostic procedures.