Invitrogen™ dNTP Mix (10 mM ea)

Catalog No.	Quantity
18-427-088	1 mL
18-427-089	5 x 1 mL
18-427-013	100 μL

Catalog No. 18-427-088 Supplier Invitrogen™ Supplier No. 18427088

Description

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes.

Invitrogen 10 mM dNTP Mix includes four nucleotides (dATP, dCTP, dGTP, dTTP) in 0.6 mM Tris-HCl. Each nucleotide is at a concentration of 10 mM and this mix is suitable for use in a wide variety of molecular processes including: polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Features include:
• Chemically synthesized
• >99% purity confirmed by HPLC
• Stable for 2 years at –20°C
• Free from qPCR, PCR, reverse transcription inhibitors
• Free of DNases and RNases
• Free of human and E. coli DNA

Applications
• qPCR, RT-qPCR
• PCR, RT-PCR, cDNA synthesis
• High-fidelity and long-range PCR
• Isothermal amplification
• DNA labeling
• Cloning
• Sanger and next-generation sequencing (NGS)

Thermo Fisher Scientific offers a wide variety of dNTPs in different concentrations, volumes, and formats.

Order Info

Approved for shipment on Wet or Dry Ice

Specifications

• Concentration: 10 mM
• Content And Storage: dNTP Mix, contains 10 mM each of dATP, dCTP, dGTP, and dTTP (1 mL)
  
  Store at –20°C.
• Form: Liquid
• Label or Dye: Unlabeled
• Quantity: 1 mL
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• For Use With (Application): Standard PCR, RT-PCR, Real Time PCR (qPCR)

Frequently Asked Questions (FAQs)

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

Why do some dNTP mixes contain dTTP and others TTP?

Thymine is normally found as a deoxyribose. Thus, TTP and dTTP are essentially the same thing. Both types of mixes can be used in PCR reactions

What concentration of dNTPs is typically used in a PCR amplification?

The four dNTPs (dATP, dCTP, dTTP, dGTP) are used at a final concentration of 200 µM each or a total of 0.8 mM in standard amplifications. For PCR amplifications that require high fidelity, lower dNTP concentrations (on the order of 10-50 µM) work optimally.

For Research Use Only. Not for use in diagnostic procedures.