Learn More
Invitrogen™ Dynabeads™ Streptavidin Magnetic Beads
Description
Includes
Supplied in PBS, pH 7.4 with 0.1% BSA and 0.02% NaN3 added as preservatives.
Dynabeads Streptavidin Magnetic Beads are designed for high-specificity, low-background capture of target molecules with biotinylated affinity binders such as antibodies, antigens, or oligonucleotides. Available in various sizes and surface chemistries, these beads can be tailored to provide the best fit for specific application needs.
Dynabeads Streptavidin Magnetic Beads are compatible with high-throughput automation platforms, including KingFisher sample purification systems, ensuring seamless integration into new and existing workflows.
Key advantages of Dynabeads Streptavidin Magnetic Beads
- ISO 13485–certified manufacturing—exceptional manufacturing control to ensure robust and consistent performance between production lots, meeting international quality standards for medical devices
- Monosized, monodispersed magnetic beads—uniform particle size provides consistent and reproducible results across applications
- Dual size options—available in 1-μm and 2.8-μm diameter specifications to accommodate diverse experimental requirements and application-specific needs
- Multiple surface chemistry options—various surface functionalization chemistries available to optimize binding efficiency and compatibility for specific applications
- Application-optimized design—size and surface chemistry combinations enable best-fit selection for targeted experimental protocols and assay requirements
Precision-engineered manufacturing delivers exceptional control over critical bead properties
Dynabeads Streptavidin products are based on magnetic polystyrene-based beads that are covalently coupled with recombinant streptavidin, creating a robust platform for target capture and isolation using biotinylated affinity binders. Dynabeads Streptavidin product manufacturing enables exceptional precision over three critical parameters: size distribution, magnetization properties, and surface chemical characteristics. This ensures consistent performance across batches and applications, providing the reliability necessary for reproducible experimental outcomes.
Optimized size selection enhances application-specific performance
The Dynabeads Streptavidin product line offers two distinct size options, each engineered to excel in specific experimental contexts. The 1-μm beads, available in the Dynabeads MyOne series, are ideally suited for applications requiring fast reaction kinetics, slow sedimentation rates, and high sensitivity-to-capacity ratios. These smaller beads provide enhanced surface area contact and reduced gravitational settling, making them particularly valuable for sensitive detection assays.
In contrast, the 2.8-μm beads, found in the Dynabeads M-270 and M-280 series, are perfect for applications demanding low background binding and reduced risk of aggregation in aggregation-prone applications. These larger beads offer even easier handling and faster magnetic separation compared to the Dynabeads MyOne series, facilitating streamlined workflow processing.
Tailored surface chemistry options address diverse experimental requirements
The Dynabeads Streptavidin product line includes two distinct surface chemistry configurations designed to optimize performance across varied experimental conditions. Hydrophobic surfaces are available in Dynabeads MyOne Streptavidin T1, Dynabeads Streptavidin for Target Enrichment, and Dynabeads M-280 Streptavidin products. These surfaces are BSA-blocked after streptavidin coupling making them ideal for high signal-to-noise applications such as immunoassays, target enrichment, and on-bead PCR for next-generation sequencing.
Alternatively, hydrophilic surfaces are featured in Dynabeads MyOne Streptavidin C1, Dynabeads Streptavidin for In Vitro Transcription, and Dynabeads M-270 Streptavidin products. These surfaces contain no BSA blocking and are particularly suitable for hydrophobic targets, harsh lysis conditions, sample preparation workflows for mass spectrometry, and applications demanding low or no nuclease activity, such as on-bead reverse transcription or in vitro transcription.
For researchers uncertain about which bead type best suits their application, a Dynabeads Streptavidin Trial Kit is available. It allows for side-by-side performance evaluation of different bead formulations under your specific experimental conditions, ensuring optimal selection for your research needs.
ISO 13485 certification ensures scalable quality and OEM support
Manufactured under ISO 13485 certification, Dynabeads Streptavidin products deliver outstanding batch-to-batch consistency. This robust quality assurance framework ensures that once customers develop an assay or workflow, they can rely on reproducible results across multiple experimental iterations. This consistency makes these versatile beads an efficient solution for applications spanning the entire spectrum from fundamental research to commercial manufacturing processes. For researchers developing commercial products or services, or those requiring larger bulk volumes, specialized support is available by emailing oemdynal@thermofisher.com.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Bead Type | Magnetic polystyrene-based beads covalently coupled with recombinant Streptavidin |
| Concentration | 10 mg/mL |
| Content And Storage | Dynabeads MyOne Streptavidin T1 are supplied in PBS, pH 7.4, with 0.1% BSA and 0.02% NaN3 . Store at 2–8°C. |
| Format | Beads in Suspension |
| Isolation Technology | Magnetic Bead |
| Diameter (Metric) | 1 μm (CV < 5%) |
| For Use With (Equipment) | KingFisher™ Sample Purification System, DynaMag™ magnets |
| For Use With (Application) | Immunoassays, IP, ChIP, RIP, cell isolations, or DNA isolation for downstream amplification reactions or NGS, requiring high sensitivity, rapid target capture with compatibility with subsequent enzymatic reactions |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | Dynabeads |
| Show More |
Frequently Asked Questions (FAQs)
This is dependent on coating or the biotinylated molecule properties. Our recommendation is that this should be tested to find optimum conditions for the specific assay.
The density of Dynabeads magnetic beads is a challenging property to determine. The reason is that Dynabeads magnetic beads have a 17-37% magnetic iron oxide content in order to have a reasonable magnetic separation time, and the density of the iron oxide is about 4.9 g/cm3. Dynabeads magnetic beads are composite materials, being a mix of polymers and iron oxide, and there are very few polymers that have a density below 1.
The sedimentation rate depends on the bead diameter squared, so the sedimentation of a 1 µm bead is much slower than that of 2.8 µm. The effect of diameter on sedimentation rate is to some extent counteracted by the fact that smaller beads need to have a higher content of iron oxide for magnetic separation applications. Typically, our M-280 Dynabeads (diameter 2.8 µm) have a density of 1.4 g DS/cm3 (DS = dry substance), our M-270 Dynabeads (diameter 2.8 µm) and M-450 Dynabeads (diameter 4.5 µm) have a density of 1.6 g DS/cm3, and our MyOne Dynabeads (diameter 1 µm) have a density of 1.8 g DS/cm3. as well as our Dynabeads Cell Isolation and Expansion Support Center and Protein Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), and Pulldown Support Center.
When exposed to a sample consisting of different types of molecules, any solid phase matrix can interact with these molecules due to hydrophobicity, charge or other types of interactions. It is not surprising that you get non-specific binding to the beads. This method is actually used for pre-clearing of sample and is not considered a good negative control. When pre-blocked and coated with a specific molecule, beads show a lot less non-specific binding than when they are not coated. As a negative control, you could try beads that are coated with an irrelevant molecule.
There are two methods to dissociate the non-biotinylated DNA from the biotinylated DNA strand. The following protocols are based on using 20 µL of Dynabeads Streptavidin, but are scalable. Both methods may release very small amounts of complementary biotinylated strand from streptavidin. If it is critical that no biotinylated strand is released, either adopt a different biotin modification using dual biotin (two biotin groups in sequence) or covalently bind DNA to e.g., Dynabeads M-270 Carboxylic Acid.
Using heat:
- Wash the DNA coated Dynabeads in 50 µL 1 x SSC.*
- Resuspend the beads in another 50 µL of 1 x SSC Incubate at 95 degrees C for 5 mins.
- Quickly put the tube in magnet stand for 1-2 mins and transfer the supernatant to a new tube.
- The supernatant contains your non-biotinylated DNA strand.
Using NaOH:
- Wash the DNA coated Dynabeads in 50 µL 1 x SSC.*
- Resuspend the beads in 20 µl of freshly prepared 0.15 M NaOH.
- Incubate at room temperature for 10 mins. Put the tube in magnet stand for 1-2 mins and transfer the supernatant to a new tube.
- The supernatant contains your non-biotinylated DNA strand. Neutralize the probe by adding 2.2 µL 10 x TE, pH 7.5 and 1.3 µL 1.25 M acetic acid.
Wash the Dynabeads coated with biotinylated strand once with 50 µL 0.1M NaOH, once with 50 µL of B&W buffer and once with 50 µL TE buffer.
*1 x SSC (0.15 M NaCl, 0.015 M sodium citrate. Dissolve the reagents in 800 mL water. Adjust pH to 7.0 with NaOH. Adjust the volume to 1 liter with water).
Assay the supernatant for unbound molecules. This will determine the amount of molecule bound to the Dynabeads. For nucleic acids, the concentration can be checked by OD readings, or by running a gel. For proteins, the concentration in the supernatant can be determined by a spectrometer using a protein assay like BCA. Alternatively, you can label the molecule with radioactivity or fluorescence and measure the concentration of molecule directly on the beads (former) or in the supernatant (latter).
For Research Use Only.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.