Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli  

Supplied at transformation efficiency of 1 x 10⁹ cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Manufacturer:  Invitrogen™ C404052

Catalog No. C404052

Description

Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
• Bacterial/Yeast Strain: TOP10

Cloning, Transformation

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Specifications

• Bacterial or Yeast Strain: TOP10
• Cloning Methylated DNA: Yes
• Competent Cell Type: Electrocompetent
• F'episome (to make ssDNA): Lacks F' Episome
• Format: One Shot
• Improves Plasmid Quality: Yes
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Product Type Specs: E. coil
• Quantity: 20 reactions
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)

• Blue/White Screening: Yes
• Cloning Unstable DNA: Not Suitable for cloning unstable DNA
• Content And Storage: Contains:
  • One Shot™ TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each
  • pUC19 DNA (10 pg/µl): 1 vial, 50 µl
  • S.O.C. medium: 1 bottle, 6 ml
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• For Use With (Application): Transformation, Cloning
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Plasmid: May be used for plasmids > 20 kb
• Product Line: One Shot™
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Reduces Recombination: Yes
• Species: E. coli
• Transformation Efficiency: > 1x10^10

For Research Use Only. Not for use in diagnostic procedures.