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Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Supplied at transformation efficiency of 1 x 109 cfu/ μg supercoiled DNA that's ideal for high-efficiency cloning and plasmid propagation

Manufacturer:  Invitrogen™ C404052

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Catalog No. C404052

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Provides stable replication of high copy number plasmids. The genotype of TOP10 cells is similar to that of DH10B™ strain.

  • hsdR for efficient transformation of unmethylated DNA from PCR amplifications
  • mcrA for efficient transformation of methylated DNA from genomic preparations
  • lacZΔM15 for blue/white color screening of recombinant clones
  • endA1 for cleaner preparations of DNA—get better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
  • recA1 for reduced occurrence of nonspecific recombination in cloned DNA
  • Bacterial/Yeast Strain: TOP10

Cloning, Transformation

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Lacks F' Episome
One Shot
Not Suitable for preparing unmethylated DNA
E. coil
20 reactions
Dry Ice
High Efficiency (> 10^9 cfu⁄µg)
Not Suitable for cloning unstable DNA
• One Shot™ TOP10 Electrocomp™ E. coli: 21 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Transformation, Cloning
Not High-throughput Compatible (Manual)
May be used for plasmids > 20 kb
One Shot™
Not for ccdB vector propagation
E. coli
> 1x10^10
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For Research Use Only. Not for use in diagnostic procedures.