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Invitrogen™ ElectroMAX™ DH10B™ Cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Ideal for efficient cloning of both prokaryotic and eukaryotic genomic DNA

Manufacturer:  Invitrogen™ 18290015

Catalog No. 18-290-015


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Includes: ElectroMAX DH10B Cells: 5 vials, 100µL each, pUC19 DNA (10pg/µL): 1 vial, 50µL, S.O.C. medium: 2 bottles, 6mL each

Description

Description

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/µg plasmid DNA. ElectroMAX DH10B Cells are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

  • High-efficiency transformation to maximize clones
  • Enhanced genetic markers for cDNA or gDNA cloning capabilities

Highest transformation efficiencies

  • ElectroMAX DH10B Cells offer the highest transformation efficiencies available with >1 x 1010 transformants/µg of control DNA in a 20 µL reaction
  • These high transformation efficiencies make ElectroMAX DH10B Cells ideal for efficient plasmid rescue from eukaryotic genomes
  • Suitable for construction of gene banks, for generation of cDNA libraries using plasmid-derived vectors, and for situations when there is limited amount of DNA
  • Versatile cloning capabilities
  • ElectroMAX DH10B Cells have the following features:
    • Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-gal or Bluo-gal
    • mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine
    • endA1 mutation enables increased plasmid yield and quantity

    Not high-throughput compatible (Manual)

    Cloning, Transformation

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    Order Info

    Shipping Condition: Dry Ice

Specifications

Specifications

Electrocompetent
ElectroMAX DH10B Cells: 5 vials, 100μL each, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 2 bottles, 6mL each
> 1x1010
Yes
Yes
Cloning, Transformation
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
DH10B
No
No
Yes
E. coli
0.5mL
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