Invitrogen™ ElectroMAX™ DH12S Cells

Description

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production of single-stranded (ss)DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries.

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production of single-stranded (ss)DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries.

Benefits of ElectroMAS DH12S Competent Cells
• Maximize yield of transformants using small amounts of DNA
• High-yield plasmid production
• Ability to create representative genomic libraries

Optimized strain for ssDNA production
The DH12S strain is endA⁺, which allows the production of ssDNA with less contamination of double-stranded (ds)DNA. The lacIq on the F´ episome allows for regulatable expression from the lac promoter. Most E. coli strains used in cloning genomic DNA have intact the systems that restrict DNA containing methylated cytosine and adenine residues (coded by mcrA, mcrB, mcrC, and mrr genes). Such wild type systems have been eliminated in the DH12S strain, allowing the construction of more representative genomic libraries and use in more efficient plasmid rescue procedures. M13KO7 helper phage is supplied for the production of ssDNA and together with DH12S cells is utilized in antibody discovery workflows (e.g., screening Ab libraries using panning method).

ElectroMAX DH12S Cells offer:
• >1 x 10¹⁰ transformants/μg efficiency with electroporation for unsurpassed cloning results
• A unique genotype to support production of exceptionally clean single stranded DNA
• Stable F´ episome to eliminate the need for growth on minimal media
• Better sequence representation eliminating mcrA, mcrBC, mrr, hsdRMS restriction systems
• Blue/white screening of recombinant clones due to lacZΔM15
• Ready-to-use M13KO7 helper phage for single stranded DNA production

Note: A High-voltage electroporation apparatus is required.

Genotype
φ80ΔlacZΔM15 mcrA Δ(mrr-hsdRMS-mcrBC) araD139 Δ(ara-leu)7697 Δ(lacX74 galU galK rpsL(Str^R) nupG recA1 / F′ [proAB+lacI^qZΔM15 Tn10(Tet^R)]

Find the strain and format that fits your needs
• DH10B Cells, the parent strain of DH12S, are available in both electrocompetent and chemically competent formats.
• MAX Efficiency DH5αF´IQ Competent Cells (Cat. No.18288019) may be used for ssDNA production and come with M13mp18 RF DNA control and are chemically competent cells.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Electrocompetent Cells
• Contains F' Episome: Yes
• Improves Plasmid Quality: No
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Content And Storage: • ElectroMAX DH12S Cells (5 x 100 μL)
  • pUC19 DNA (50 μL at 10 pg/μL)
  • M13KO7 Helper Phage (2 x 1 mL)
  Store at –80°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: Yes (Streptamycin, Tetracycline)
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Plasmid: May be used for plasmids >20 kb
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Promoter: Laclq
• Format: Tube
• Product Line: ElectroMAX™
• Quantity: 5 x 100 μL

For Research Use Only. Not for use in diagnostic procedures.