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Invitrogen™ ElectroMAX™ DH12S™ Cells Non-distribution product as customer accommodation.

Useful for production of single-stranded DNA

Manufacturer:  Invitrogen™ 18312017

Catalog No. 18-312-017


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Description

Description

ElectroMAX DH12S Cells, a derivative of DH10B are suitable for transformation by electroporation. They may be used in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries. The DH12S strain is endA+, which allows the production of ssDNA with less contaminating double-stranded DNA. The lacIq on the F' marker allows for regulatable expression from the lac promoter. Unlike most E. colihosts used in cloning genomic DNA, the systems that restrict DNAs containing methylated cytosine and adenine residues (mcrA, mcrB, mcrC, and mrr) have been eliminated in
the DH12S strain, allowing the construction of more representative genomic libraries and more efficient plasmid rescue procedures. M13KO7 helper phage is also supplied for the production of ss DNA.

  • >1 x 1010 transformants/µg efficiency with electroporation for unsurpassed cloning results
  • Unique genotype to support production of exceptionally clean single stranded DNA
  • Stable F' episome to eliminate the need for growth on minimal media
  • Better sequence representation eliminating mcrA, mcrBC, mrr, hsdRMS restriction systems
  • Blue/white screening of recombinant clones due to lacZΔM15
  • Ready-to-use M13KO7 helper phage for single stranded DNA production
  • High efficiency (> 109 cfu/µg)

Not high-throughput compatible (Manual)

Cloning, Transformation

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Shipping Condition: Dry Ice

Specifications

Specifications

Electrocompetent
Yes
No
Yes
E. coli
0.5mL
DH12S
> 1x1010
No
Yes
Cloning, Transformation
Store in ultra-cold freezer (-68 to -85°C).
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For Research Use Only. Not for use in diagnostic procedures.