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Invitrogen™ ElectroMAX™ DH5α-E Competent Cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Suitable for transformation by electroporation or in procedures requiring high transformation efficiencies

Manufacturer:  Invitrogen™ 11319019

Catalog No. 11-319-019


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Includes: ElectroMAX DH5α-E Competent Cells: 5 vials, 100µL each, pUC19 DNA (10pg/µL): 1 vial, 50µL, S.O.C. medium: 2 bottles, 6mL each

Description

Description

ElectroMAX DH5α-E™ Competent Cells are derived from the DH5α strain. They may be used in procedures such as generation of cDNA libraries or in transformations with limited input DNA.

  • >1 x 1010 transformants/µg efficiency with electroporation
  • Greatly increased plasmid yield and quality due to endA1 mutation
  • High-efficiency transformation with plasmids 30 kb in size
  • Blue/white screening of recombinant clones due to lacZΔM15
  • Ensured insert stability due to recA1 mutation

Not high-throughput compatible (Manual)

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

DH5α
Yes
Electrocompetent
Lacks F' Episome
May be used for plasmids > 20 kb
DH5a, ElectroMAX™
Yes
No
> 1x10^10
Yes
Yes
Not Suitable for cloning unstable DNA
Contains:
• ElectroMAX DH5α-E Competent Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Not High-throughput Compatible (Manual)
Not Suitable for preparing unmethylated DNA
Not for ccdB vector propagation
Dry Ice
High Efficiency (> 10^9 cfu⁄µg)
Tube(s)
E. coli
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For Research Use Only. Not for use in diagnostic procedures.