Invitrogen ElectroMAX DH5α-E Competent Cells
Suitable for transformation by electroporation or in procedures requiring high transformation efficiencies
Manufacturer: Invitrogen 11319019
ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain. They may be used in procedures such as generation of cDNA libraries or in transformations with limited input DNA.
- >1 x 1010 transformants/µg efficiency with electroporation
- Greatly increased plasmid yield and quality due to endA1 mutation
- High-efficiency transformation with plasmids 30 kb in size
- Blue/white screening of recombinant clones due to lacZΔM15
- Ensured insert stability due to recA1 mutation
Not high-throughput compatible (Manual)
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Shipping Condition: Dry Ice
|Lacks F' Episome|
|May be used for plasmids > 20 kb|
|Not Suitable for cloning unstable DNA|
• ElectroMAX DH5α-E Competent Cells: 5 vials, 100 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
|Not High-throughput Compatible (Manual)|
|Not Suitable for preparing unmethylated DNA|
|Not for ccdB vector propagation|
|High Efficiency (> 10^9 cfu⁄µg)|
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For Research Use Only. Not for use in diagnostic procedures.