Invitrogen™ pAd/CMV/V5-DEST™ Gateway™ Vector Kit

Description

Includes

pAd-CMV/V5-DEST Vector 150ng/μL in TEBuffer, pH 8.0. 40μL; pAd/CMV/V5-GW/lacZ control plasmid 1μg/μL in TE Buffer, pH 8.0, 10μL.

For easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and nondividing mammalian cells

• The vector allows generation of an adenovirus containing the target gene where constitutive, high-level expression is driven by the CMV promoter
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and nondividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications
• CMV promoter for high-level constitutive expression of gene of interest
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Herpes Simplex Virus thymidine kinase (TK) polyadenylation sequence for efficient transcription termination and polyadenylation of mRNA
• V5 epitope for detection of recombinant protein
• Ampicillin selection marker

Antibody and Cell Culture Production, Bioproduction, Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery, Viral Vaccine Production

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Adenoviral
• Promoter: CMV
• Product Type: Mammalian Expression Vector
• Content And Storage: • pAd-CMV⁄V5-DEST™ Vector 150 ng⁄μl in TEBuffer, pH 8.0. 40 μl (Store at -20°C)
  • pAd⁄CMV⁄V5-GW⁄lacZ control plasmid 1 μg⁄μl in TE Buffer, pH 8.0. 10 μl (Store at -20°C)
• Protein Tag: V5 Epitope Tag
• Cloning Method: Gateway
• Quantity: 6 μg
• Vector: pDEST
• Product Line: Gateway™, ViraPower™
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.