Invitrogen™ E. coli DNA Ligase

Description

Includes

10X Reaction Buffer [188mM Tris-HCl (pH 8.3), 906mM KCl, 46mM MgCl₂, 37.5mM DTT, 1.5mM λ-NAD, 100mM (NH₄ )₂ SO₄ ]

Catalyzes formation of phosphodiester bonds in presence of β-NAD between double-stranded DNAs with 3' hydroxyl and 5' phosphate cohesive termini

Single-stranded nucleic acids are not substrates for this enzyme.

• Source: Purified from E. coli 594 (Su^-) bearing λ lysogen gt4lop-11 lig⁺ S7
• Performance and Quality Testing: Endodeoxyribonuclease and 3' and 5' exodeoxyribonuclease assays; ligation efficiency tested
• Unit Definition: One unit is amount of enzyme required to give 50% ligation of Hind III-digested λ DNA in 30 min. at 16°C in a final volume of 20μL containing a 5' termini concentration of 0.12 'M (300μg/mL)
• Unit Reaction Conditions: 18.8mM Tris-HCl (pH 8.3), 90.6mM KCl, 4.6mM MgCl₂ , 3.8mM DTT, 0.15mM λ-NAD, 10mM (NH₄ )₂ SO₄ in 20μL for 1 hr. at 16°C

Second-strand cDNA synthesis; T4 DNA Ligase alternative when blunt-end ligation is not required; Cloning, Restriction Enzyme Cloning

Order Info

Shipping Conditions: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: E. coli DNA Ligase is supplied with a vial of 10X reaction buffer [188 mM Tris-HCl (pH 8.3), 906 mM KCl, 46 mM MgCl₂ , 37.5 mM
  DTT, 1.5 mM λ-NAD, 100 mM (NH₄ )₂ SO₄ ]. Store at -20°C.
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Enzyme: Ligase
• Compatible Buffer: 10X Reaction Buffer
• Quantity: 100 U
• Product Type: DNA Ligase

For Research Use Only. Not for use in diagnostic procedures.