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Invitrogen™ Taq DNA Polymerase, recombinant

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Manufacturer:  Invitrogen™ 10342053

Encompass

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Catalog No. 10-342-053


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Description

Description

The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity.

  • Choice of recombinant or native enzyme
  • Amplification of PCR products up to 5kb in size
  • An enzyme that is licensed and qualified for PCR
  • Source: Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli
  • Applications: Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
  • Unit definition: One unit of TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C

Specifications

Specifications

Contains:
• 20 μL Taq DNA Polymerase (5 U/μL)
• 1.25 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
• 1 mL Magnesium Chloride (50 mM)

Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.
Primer-Probe
Stand-alone enzyme
No
3'-A
Taq DNA Polymerase
Standard
DNA
DNA Polymerase
Approved for shipment on Wet or Dry Ice
5 U/µL
1 X
Low
100 Reactions
RT-PCR
Separate Components
5 kb or less
5' - 3'
100 Units
SDS
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For Research Use Only. Not for use in diagnostic procedures.