Invitrogen™ Taq DNA Polymerase, recombinant

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Supplier:  Invitrogen™ 10342053

Catalog No. 10-342-053

Includes: Taq DNA Polymerase (5U/μL); 10X PCR buffer (200mM Tris-HCl pH 8.4, 500mM KCl); Magnesium Chloride (50mM)

Description

The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It has a 5'-3' DNA polymerase activity and a 5'-3' exonuclease activity.

• Choice of recombinant or native enzyme
• Amplification of PCR products up to 5kb in size
• An enzyme that is licensed and qualified for PCR
• Source: Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli
• Applications: Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
• Unit definition: One unit of TaqDNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C

Order Info

Shipping Condition: Approved for shipment on wet or dry ice

Specifications

• Content And Storage: Contains:
  • 20 μL Taq DNA Polymerase (5 U/μL)
  • 1.25 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
  • 1 mL Magnesium Chloride (50 mM)
  
  Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.
• Format: Stand-alone enzyme
• PCR Method: qPCR, Standard PCR
• Reaction Speed: Standard
• Product Type: DNA Polymerase
• Shipping Condition: Approved for shipment on Wet or Dry Ice
• Fidelity (vs. Taq): 1 X
• No. of Reactions: 100 Reactions
• Reaction Format: Separate Components
• Starting Material: DNA

• Detection Method: Primer-probe
• GC-Rich PCR Performance: Low
• Polymerase: Taq DNA Polymerase
• Exonuclease Activity: 5' - 3'
• Quantity: 100 Units
• Concentration: 5 U/µL
• Hot Start: No
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

For Research Use Only. Not for use in diagnostic procedures.