Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL)

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

Supplier:  Invitrogen™ 28025021


 View more versions of this product

Catalog No. 28-025-021

Add to Cart



Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
  • Thermostability—optimal activity at 37°C
  • Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
  • Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
  • Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200U/μL
  • Unit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
  • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice



• M-mLV RT, 1 mL (200 U/μL)
• First Strand Buffer, 4 x 1 mL
• DTT, 4 x 500 μL (100 mM)

Store at –20°C.

50 min.
200 U/μL
1,000 Reactions
Reverse Transcription
Stand-alone Enzyme
Reverse Transcription
Wet Ice
First-Strand cDNA
Separate components
Up to 7 kb


Product Certifications


Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only. Not for use in diagnostic procedures.