Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL)

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

Supplier:  Invitrogen™ 28025021

Catalog No. 28-025-021

Description

Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.

• Thermostability—optimal activity at 37°C
• Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
• Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
• Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
• Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
• Concentration: 200U/μL
• Unit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)₂₅ as template-primer
• Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl₂, 1mM DTT, 0.5mM [³H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)₂₅, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
• Optimal reaction temperature: 37°C
• Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice

Specifications

• Content And Storage:

  • M-mLV RT, 1 mL (200 U/μL)
  • First Strand Buffer, 4 x 1 mL
  • DTT, 4 x 500 μL (100 mM)
  
  Store at –20°C.
  
  

• Reaction Speed: 50 min.
• Optimal Reaction Temperature: 37°C
• Ribonuclease H Activity: Yes
• Concentration: 200 U/μL
• No. of Reactions: 1,000 Reactions
• Reagent Type: Reverse Transcription
• Starting Material: RNA

• Format: Stand-alone Enzyme
• Technique: Reverse Transcription
• Reverse Transcriptase: M-MLV
• Shipping Condition: Wet Ice
• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Size (Final Product): Up to 7 kb

For Research Use Only. Not for use in diagnostic procedures.