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Invitrogen™ M-MLV Reverse Transcriptase (200 U/µL)

Recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid

$334.00 - $1508.00

Specifications

Concentration 200 U/μL
Final Product Type cDNA (First-Strand)
Format Stand-alone enzyme
GC-Rich PCR Performance Low
PCR Method RT-PCR, RT-qPCR
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Products 2
Catalog Number Mfr. No. Includes Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Includes Quantity Price Quantity & Availability  
28-025-013
Encompass
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Invitrogen™
28025013
1 x 200μL M-MLV RT (200U/μL); 1mL 5X First Strand Buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM Magnesium Chloride]; 500μL 100mM DTT; 40,000 units 200 Reactions; 40,000 units
Each for $334.00
Add to cart
 
28-025-021
Encompass
View Documents
Invitrogen™
28025021
1000 Reactions
Each for $1,508.00
Add to cart
 
Description

Description

Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity.
  • Thermostability—optimal activity at 37°C
  • Size of cDNA—M-MLV can be used to synthesize first-strand cDNA up to 7kb
  • Applications—synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR
  • Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid
  • Performance and quality testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
  • Concentration: 200U/μL
  • Unit definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1nmole of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)25 as template-primer
  • Unit reaction conditions: 50mM Tris-HCl (pH 8.3), 40mM KCl, 6mM MgCl2, 1mM DTT, 0.5mM [3H]dTTP, 0.1mM poly(A), 0.1mM oligo(dT)25, 0.1mg/mL BSA, and enzyme in 50μL for 10 minutes at 37°C
  • Optimal reaction temperature: 37°C
  • Final product size: 7kb or less

PCR & Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet ice

Specifications

Specifications

200 U/μL
Stand-alone enzyme
RT-PCR, RT-qPCR
Standard
RNA
RNA
RNA dependent DNA Polymerase
M-MLV Reverse Transcriptase
Reduced
Wet Ice
cDNA (First-Strand)
Low
Separate components
Reverse Transcription
7 kb or Less
Reverse Transcription
37°C
M-MLV
Low
ssDNA, ssRNA, RNA-DNA Hybrid
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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