Invitrogen™ MAX Efficiency™ DH10B Competent Cells

Description

Includes

MAX Efficiency DH10B Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, S.O.C. medium: 2 bottles, 6mL each

MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.

MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency, making them ideal for generating plasmid libraries, an ability to take up and maintain large plasmids (seen with plasmids of 150 kb in size), and a lack of methylation-dependent restriction systems.

Benefits of MAX Efficiency DH10B Competent Cells
• Ability to maintain large DNA plasmids 
• Resistant to the effects of ligase and ligase buffer and tolerant of small amounts of undiluted ligation reactions 
• Suitable for the construction of cDNA or genomic DNA libraries using plasmid-derived vectors 

Universal strain for everyday applications
The presence of the mcrA genotypic marker and the deletion of mcrBC and mrr makes this strain suitable for cloning DNA that contains methylcytosine and methyladenine. Therefore, genomic DNA, both prokaryotic and eukaryotic, can be cloned efficiently in DH10B.

Versatile DH10B genotype features
• lacZ∆M15 for blue/white screening of recombinant clones 
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries 
• endA1 mutation to increase plasmid yield and quantity 

Genotype
F–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara-leu)7697 galU galK λ–rpsL(StrR) nupG

Find the strain and format that fits your need 
• DH10B Cells are available in both electrocompetent and chemically competent formats.
• MegaX DH10B T1^R Electrocomp Cells (Cat. No.C640003) have the highest transformation efficiency (>3 x 10¹⁰ cfu/μg plasmid DNA).
• Multishot formats are available for high throughput applications.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Content And Storage: • MAX Efficiency DH10B Competent Cells (5 x 200 μL)
  Store Competent Cells at –70°C
  
  • pUC19 DNA (100 μL at 0.01 μg/mL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. Medium (2 x 6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Format: Tube
• Product Line: MAX Efficiency™
• Quantity: 5 x 200 μL

For Research Use Only. Not for use in diagnostic procedures.