Invitrogen™ One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Description

One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection.

One Shot MAX Efficiency DH10B T1 Phage-Resistant E. coli have all the benefits of the DH10B genotype, with the addition of the tonA (also known as fhuA) genotype for resistance to T1 and T5 phage infection. The inclusion of the tonA genotype offers added security to protect valuable clones and libraries. MAX Efficiency DH10B T1 cells can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid, making them ideal for generating cDNA or genomic libraries, transforming large plasmids (>150 kb in size), and for situations when DNA is limiting.

MAX Efficiency DH10B T1 Phage-Resistant Cells carry mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr), allowing construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B-T1R cells an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

MAX Efficiency DH10B T1 Phage-Resistant Cells offer:
• Transformation efficiencies of >1 x 10⁹ cfu/μg
• tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• lacZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• mcrA genotypic marker and the mcrBC, mrr deletion that allows for cloning DNA that contains methylcytosine and methyladenine
• endA1 mutation that enables increased plasmid yield and quantity

Convenient and efficient format 
DH10B-T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles, or money wasted on unused cells.

Genotype
F^–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG tonA 
 
Find the strain and format that fit your needs
• We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
• The DH10B-T1R strain is available in Multishot format for high throughput applications.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Content And Storage: • One Shot MAX Efficiency DH10B T1 Phage-Resistant Cells (21 x 50 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (50 μL at 10 pg/μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. Medium (6 ml)
  Store S.O.C. Medium at 4°C or room temperature.
  
  .
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Plasmid: High Copy Plasmid
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): Yes
• Species: E. coli (K12)
• Format: Tube
• Product Line: One Shot™
• Quantity: 21 x 50 μL

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.