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Invitrogen™ One Shot™ MAX Efficiency™ DH10B T1 Phage-Resistant Cells

Ideal for generating cDNA or genomic libraries due to high transformation efficiency

Manufacturer:  Invitrogen™ 12331013


Catalog No. 12-331-013

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DH10B T1 Phage-Resistant E. colihave all the benefits of the DH10B genotype, with the addition of the tonA genotype which confers resistance to T1 and T5 phage infection. The inclusion of the tonA genotype protects your valuable samples and minimizes the possibility of downtime in your lab due to phage contamination.

  • tonA genotype to confer resistance to T1 and T5 phage
  • Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
  • The endA1 mutation to increase plasmid yield and quantity
  • ZΔM15 for blue/white screening of recombinant clones
  • DH10B-T1R E. coliare available in both electrocompetent and chemically competent formats
  • High efficiency transformation is seen with plasmids 150 kb in size

Not high-throughput compatible (Manual)

Cloning, Transformation

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Competent Cell
High Efficiency (>10^9 cfu/µg)
High Efficiency (> 10^9 cfu⁄µg)
Not Suitable for cloning unstable DNA
Not Suitable for preparing unmethylated DNA
E. coli
One Shot™
Lacks F' Episome
Chemically Competent
Store in ultra-cold freezer (-68 to -85°C).
Not High-throughput Compatible (Manual)
Not for ccdB vector propagation
One Shot
Product Certifications


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For Research Use Only. Not for use in diagnostic procedures.