Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells  

High-efficiency chemically competent cells specifically designed for cloning unstable inserts

Manufacturer:  Invitrogen™ 10268019

Catalog No. 10-268-019

Includes: MAX Efficiency Stbl2 Competent Cells: 5 vials, 200µL each (total of 1mL), pUC19 DNA (0.01µg/mL): 1 vial, 100µL, SOC Medium: 1 bottle, 6mL

Description

In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes.

• 1 x 10⁹ transformants/µg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• Unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation

Not High Throughput-Compatible (Manual)

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

• Bacterial or Yeast Strain: Stbl2™
• Cloning Methylated DNA: Yes
• Competent Cell Type: Chemically Competent
• F'episome (to make ssDNA): Lacks F' Episome
• Plasmid: May be used for plasmids > 20 kb
• Product Line: MAX Efficiency™, Stbl2™
• Reduces Recombination: Yes
• T1 Phage - Resistant (tonA): No
• Transformation Efficiency: > 1x10^9
• Format: Tube(s)

• Blue/White Screening: No
• Cloning Unstable DNA: Suitable for cloning unstable DNA
• Content And Storage: Contains:
  • MAX Efficiency™ Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
  • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
  • SOC Medium: 1 bottle, 6 ml
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Shipping Condition: Dry Ice
• Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
• Species: E. coli
• Improves Plasmid Quality: Yes

For Research Use Only. Not for use in diagnostic procedures.