Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.

Supplier:  Invitrogen™ 10268019

Catalog No. 10-268-019

Includes: MAX Efficiency Stbl2 Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, SOC Medium: 1 bottle, 6mL

Description

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. The Stbl2 strain was used for the stable propagation of a plasmid containing approximately 100 direct repeats of 32 bp as well as full-length infectious simian immunodeficiency virus (SIV) clone can be maintained. This strain has also been demonstrated to stably propagate plant virus sequences such as potato virus Y (PVY).

MAX Efficiency Stbl2 Competent Cells are a derivative of the JM109/J5 strain. Competent cells are prepared by a patented modification of the procedure of Hanahan. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow for the cloning of methylated genomic sequences.

MAX Efficiency Stbl2 Competent Cells offer:
• >1 x 10⁹ transformants/μg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA that allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeats and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA

Genotype
F^- mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 lon gyrA96 thi supE44 relA1 λ- Δ(lac-proAB)

Note: For optimal performance, expression in S.O.C. Medium and incubation on antibiotic plates at 30°C is recommended.

Find the strain and format that fits your needs
• A variety of Stbl strains are available with different genetic background or suitable for electroporation.
• The Stbl3 strain is available in MultiShot format for high throughput applications.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Improves Plasmid Quality: Yes (endA1)
• Transformation Efficiency Level: High Efficiency (>1 x 10⁹ cfu/μg)
• Antibiotic Resistance Bacterial: No
• Blue/White Screening: No
• Plasmid: May be used for plasmids >20 kb
• Reduces Recombination: Yes (recA1)
• T1 Phage - Resistant (tonA): No
• Format: Tube
• Quantity: 5 x 200 μL

• Contains F' Episome: No
• Cloning Methylated DNA: Yes (mcrB, mrr)
• Content And Storage: • MAX Efficiency Stbl2 Competent Cells (5 x 200 μL); store at –80°C
  • pUC19 DNA (100 μL at 0.01 μg/mL); store at –20°C
  • S.O.C. Medium (6 mL); store at 4°C or room temperature
  
  
• Cloning Unstable DNA: Yes (recA1)
• High-throughput Compatibility: Low
• Preparing Unmethylated DNA: No
• Shipping Condition: Dry Ice
• Species: E. coli (K12)
• Product Line: MAX Efficiency™

For Research Use Only. Not for use in diagnostic procedures.