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Invitrogen MAX Efficiency Stbl2 Competent Cells
High-efficiency chemically competent cells specifically designed for cloning unstable inserts
Manufacturer: Invitrogen 10268019
Includes: MAX Efficiency Stbl2 Competent Cells: 5 vials, 200µL each (total of 1mL), pUC19 DNA (0.01µg/mL): 1 vial, 100µL, SOC Medium: 1 bottle, 6mL
Description
In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes.
- 1 x 109 transformants/µg plasmid DNA for cloning rare sequences
- Elimination of mcrBC-hsdRMS-mrr and mutation of mcrAallows cloning of methylated genomic sequences
- Unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
- Greatly increased plasmid yield and quality due to endA1 mutation
Not High Throughput-Compatible (Manual)
Cloning, Transformation
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Order Info
Shipping Condition: Dry Ice
Specifications
Stbl2™ | |
Yes | |
Chemically Competent | |
Lacks F' Episome | |
May be used for plasmids > 20 kb | |
MAX Efficiency™, Stbl2™ | |
Yes | |
No | |
> 1x10^9 | |
Tube(s) |
No | |
Suitable for cloning unstable DNA | |
Contains: • MAX Efficiency™ Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl • SOC Medium: 1 bottle, 6 ml Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |
|
Not High-throughput Compatible (Manual) | |
Not Suitable for preparing unmethylated DNA | |
Not for ccdB vector propagation | |
Dry Ice | |
High Efficiency (> 10^9 cfu⁄µg) | |
E. coli | |
Yes |
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For Research Use Only. Not for use in diagnostic procedures.