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Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells
Description
Includes
MAX Efficiency Stbl2 Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, SOC Medium: 1 bottle, 6mL
MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts. In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes. The Stbl2 strain was used for the stable propagation of a plasmid containing approximately 100 direct repeats of 32 bp as well as full-length infectious simian immunodeficiency virus (SIV) clone can be maintained. This strain has also been demonstrated to stably propagate plant virus sequences such as potato virus Y (PVY).
MAX Efficiency Stbl2 Competent Cells are a derivative of the JM109/J5 strain. Competent cells are prepared by a patented modification of the procedure of Hanahan. The mcrA mutation and the mcrBC-hsdRMS-mrr deletion allow for the cloning of methylated genomic sequences.
MAX Efficiency Stbl2 Competent Cells offer:
• >1 x 109 transformants/μg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA that allows cloning of methylated genomic sequences
• A unique genotype that stabilizes direct repeats and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation
• recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Genotype
F- mcrA Δ(mcrBC-hsdRMS-mrr) recA1 endA1 lon gyrA96 thi supE44 relA1 λ- Δ(lac-proAB)
Note: For optimal performance, expression in S.O.C. Medium and incubation on antibiotic plates at 30°C is recommended.
Find the strain and format that fits your needs
• A variety of Stbl strains are available with different genetic background or suitable for electroporation.
• The Stbl3 strain is available in MultiShot format for high throughput applications.
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Product Type | Chemically Competent Cells |
| Contains F' Episome | No |
| Improves Plasmid Quality | Yes (endA1) |
| Cloning Methylated DNA | Yes (mcrB, mrr) |
| Transformation Efficiency Level | High Efficiency (>1 x 109 cfu/μg) |
| Content And Storage | • MAX Efficiency Stbl2 Competent Cells (5 x 200 μL); store at –80°C • pUC19 DNA (100 μL at 0.01 μg/mL); store at –20°C • S.O.C. Medium (6 mL); store at 4°C or room temperature |
| Antibiotic Resistance Bacterial | No |
| Cloning Unstable DNA | Yes (recA1) |
| Blue/White Screening | No |
| High-throughput Compatibility | Low |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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