Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

High-efficiency chemically competent cells specifically designed for cloning unstable inserts

Manufacturer:  Invitrogen™ 10268019

Catalog No. 10-268-019

Includes: MAX Efficiency Stbl2 Competent Cells: 5 vials, 200μL each (total of 1mL), pUC19 DNA (0.01μg/mL): 1 vial, 100μL, SOC Medium: 1 bottle, 6mL

Description

In addition to recA1, a unique set of genetic markers allow for stable cloning of direct repeat and retroviral sequences and tandem array genes.

• 1 x 10⁹ transformants/μg plasmid DNA for cloning rare sequences
• Elimination of mcrBC-hsdRMS-mrr and mutation of mcrA—allows cloning of methylated genomic sequences
• Unique genotype that stabilizes direct repeat and retroviral sequences such as SIV and HIV
• Greatly increased plasmid yield and quality due to endA1 mutation

Not High Throughput-Compatible (Manual)

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Competent Cell
• Efficiency: High Efficiency (>10^9 cfu/µg)
• Improves Plasmid Quality: Yes
• Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
• Cloning Unstable DNA: Suitable for cloning unstable DNA
• Blue/White Screening: No
• Plasmid: May be used for plasmids > 20 kb
• Propagating ccdB Vectors: Not for ccdB vector propagation
• Shipping Condition: Dry Ice
• Species: E. coli
• Product Line: MAX Efficiency™, Stbl2™

• Contains F' Episome: Lacks F' Episome
• Cell Type: Chemically Competent
• Cloning Methylated DNA: Yes
• Content And Storage: Contains:
  • MAX Efficiency™ Stbl2™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
  • pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
  • SOC Medium: 1 bottle, 6 ml
  
  Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• Bacterial or Yeast Strain: Stbl2™
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
• Reduces Recombination: Yes
• T1 Phage - Resistant (tonA): No
• Format: Tube
• Quantity: 5 x 200 μL

For Research Use Only. Not for use in diagnostic procedures.