Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli

Description

Includes

MultiShot TOP10 Chemically Competent E. coli: Five 96-well plates, 15μL per well, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 5 bottles, 15mL each

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains. Packaged in five 96-well microtiter plates (15 μL aliquots) to simplify high-throughput workflows and provide transformation efficiencies of >1 x 10⁸ cfu/μg control plasmid DNA, Multishot TOP10 is a versatile strain that can be used in multiple routine cloning applications.

Benefits of MultiShot TOP10 competent cells
• TOP10 strain is ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design is convenient for automated high-throughput cloning
• mcrA mutation supports efficient transfection of methylated DNA
• lacZΔM15 allele permits rapid blue/white screening of transfectants
• endA1 mutation reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation helps reduce unwanted recombination

Easy to use for high-throughput transformations
MultiShot TOP10 Chemically Competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Using our TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected. 

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that fits your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.

Order Info

Shipping Condition: Dry Ice

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: No
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (mcrA)
• Transformation Efficiency Level: Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)
• Content And Storage: • MultiShot™ TOP10 Chemically Competent E. coli (5 x 96-well plates at 15 μL per well)
  Store Competent Cells at –80°C.
  
  • pUC19 DNA (1 x 50 μL)
  Store pUC19 DNA at –20°C.
  
  • S.O.C. medium (5 x 15 mL/bottle)
  Store S.O.C. Medium at 4°C or at room temperature
• Antibiotic Resistance Bacterial: Yes (Streptomycin)
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: High
• Plasmid: High Copy Plasmid
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Format: 96-well Plate
• Product Line: MultiShot™
• Quantity: 5 x 96-well Plate (15 μL/Well)

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.