Invitrogen™ One Shot™ INVαF' Chemically Competent E. coli

Description

One Shot INVαF' Chemically Competent E. coli are designed to stably replicate high-copy number plasmids.

One Shot INVαF´ Chemically Competent E. coli are designed to stably replicate high-copy number plasmids. They allow for the preparation of high quality plasmid DNA with less carry over contaminants as compared to other popular strains such as JM109 and can achieve a transformation efficiency of >1 x 10⁸ cfu/μg supercoiled plasmid.

INVαF´ cells carry the F´ episome and so can be utilized to produce single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. This strain is related to DH strains and features similar genetic traits and benefits. The lacZΔM15 marker provides α-complementation of the ß-galactosidase gene from pUC or similar vectors and can be used for blue-white screening of colonies on bacterial plates containing Bluo-Gal or X-Gal. In addition to supporting blue/white screening, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA.

One Shot INVαF´ Chemically Competent E. coli cells offer:
• Transformation efficiencies of >1 x 10⁸ cfu/μg
• Stability and exceptional quality of high-copy number plasmid DNAb
• Blue-white screening of recombinant clones (lacZΔM15)
• Reduction of homologous recombination of transformed plasmids (recA1)
• Increased quality of plasmid DNA preparations (endA1)
• Production and rescue of ssDNA from vectors carrying the f1 origin of replication (F´)

Note: INVαF´ cells do not require IPTG to induce expression from the lac promoter.

Convenient and efficient format
One Shot INVαF´ Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one complete transformation, reducing freeze-thaw cycles and limiting money wasted on unused cells.

Genotype
F´ endA1 recA1 hsdR17 (r_K^–, m_K⁺) supE44 thi-1 gyrA96 relA1 φ80lacZΔM15 Δ(lacZYA-argF)U169 λ^–
Genetic marker descriptions

Find the strain and format that fit your needs
• A variety of DH strains are available in chemically competent and electrocompetent cell formats.
• We offer other strains for ssDNA production, such as TOP10F´ (Cat. No. C303003) or electrocompetent DH12S (Cat. No. 18312017).
• MultiShot formats are available for high throughput applications.

Specifications

• Product Type: Chemically Competent Cells
• Contains F' Episome: Yes
• Improves Plasmid Quality: Yes (endA1)
• Cloning Methylated DNA: Yes (hsd)
• Transformation Efficiency Level: Medium Efficiency (1 x 10⁸ to 1 x 10⁹ cfu/μg)
• Content And Storage: • One Shot INVαF' Chemically Competent E. coli (21 x 50 μL)
  Store Competent Cells at –80°C.
  
  • pUC19 plasmid (50 μL at 10 pg/μL)
  Store pUC19 plasmid at –20°C.
  
  • S.O.C. Medium (6 mL)
  Store S.O.C. Medium at 4°C or room temperature.
  
  
• Antibiotic Resistance Bacterial: No
• Cloning Unstable DNA: Not suitable for cloning unstable DNA
• Blue/White Screening: Yes (lacZΔM15)
• High-throughput Compatibility: Low
• Plasmid: High Copy Plasmid
• Preparing Unmethylated DNA: No
• Reduces Recombination: Yes (recA1)
• Shipping Condition: Dry Ice
• T1 Phage - Resistant (tonA): No
• Species: E. coli (K12)
• Format: Tube
• Product Line: One Shot™
• Quantity: 21 x 50 μL/tube

For Research Use Only. Not for use in diagnostic procedures.