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Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

Added security to protect valuable clones and libraries

Manufacturer:  Invitrogen™ 12297016


Catalog No. 12-297-016

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Includes: One Shot MAX Efficiency DH5α-T1R Competent Cells: 20 vials, 50μL each ( total of 1mL), pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 1 bottle, 6mL



One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain. DH5α-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

  • Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
  • Reduced occurrence of unwanted recombination in cloned DNA (recA1)
  • Efficient transformation of unmethylated DNA from PCR applications (hsdR)
  • Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)
  • DH5α-T1R E. coli are supplied in the convenient, single-reaction One Shot format
  • Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells

Not high-throughput compatible (Manual)

Cloning, Transformation

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Shipping Condition: Dry Ice



Competent Cell
High Efficiency (>10^9 cfu/µg)
High Efficiency (> 10^9 cfu⁄µg)
Not Suitable for cloning unstable DNA
Not Suitable for preparing unmethylated DNA
E. coli
One Shot™
Lacks F' Episome
Chemically Competent
• One Shot MAX Efficiency DH5α-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Not High-throughput Compatible (Manual)
Not for ccdB vector propagation
One Shot
20 x 50 μL
Product Certifications


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For Research Use Only. Not for use in diagnostic procedures.