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Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Added security to protect valuable clones and libraries

Manufacturer:  Invitrogen™ 12297016

Catalog No. 12-297-016


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Includes: One Shot MAX Efficiency DH5α-T1R Competent Cells: 20 vials, 50μL each ( total of 1mL), pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 1 bottle, 6mL

Description

Description

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain. DH5α-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.

  • Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
  • Reduced occurrence of unwanted recombination in cloned DNA (recA1)
  • Efficient transformation of unmethylated DNA from PCR applications (hsdR)
  • Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)
  • DH5α-T1R E. coli are supplied in the convenient, single-reaction One Shot format
  • Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells

Not high-throughput compatible (Manual)

Cloning, Transformation

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Order Info

Shipping Condition: Dry Ice

Specifications

Specifications

Competent Cells
E. coli
Chemically Competent
DH5α
One Shot™
Lacks F' Episome
> 1x10^9
Yes
High Efficiency (> 10^9 cfu⁄µg)
Yes
20 x 50 µL
No
Not High-throughput Compatible (Manual)
Yes
Not Suitable for cloning unstable DNA
Yes
Not Suitable for preparing unmethylated DNA
Not for ccdB vector propagation
One Shot
Safety and Handling

Safety and Handling

SDS
Documents
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For Research Use Only. Not for use in diagnostic procedures.